EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of a mutation in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs


, : Identification of a mutation in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs. Infection and Immunity 63(12): 4924-4927

We used transposon (TnphoA) mutagenesis to study the role of virulence factors of pathogenic Escherichia coli strains associated with septicemia in calves and piglets. We have produced an avirulent and serum-sensitive mutant of wild-type pathogenic strain 5131 O115:K"V165":F165 and have localized and identified the TnphoA insertion in the pstC gene of the pst-phoU operon. This operon encodes the PstSCAB transporter and PhoU protein that negatively regulate the phosphate (Pho) regulon. This mutation is pleiotropic and could have an effect on pathogenicity and on the production of the surface polysaccharides of strain 5131. The mutant demonstrated restored repressibility of alkaline phosphatase and regained the capacity to resist serum and to survive systemically for at least 5 days in experimentally inoculated pigs when complemented with plasmid pAN92, bearing the pst-phoU operon.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863184

PMID: 7591158

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Klinge, V., 1976: Relationship between basal body temperature and body activity in a human following a bilateral oophorectomy. Continuous measures of arm movement activity and basal body temperature were obtained on 1 female over a total of 149 days, beginning 3 mo. subsequent to this female's bilateral oophorectomy. Prior to the oophorectomy the subject had been men...

Sorensen, P.; Ladd, J. N.; Amato, M., 1996: Microbial assimilation of 14C of ground and unground plant materials decomposing in a loamy sand and a clay soil. The influence of grinding plant materials on the microbial decomposition and the distribution of plant-derived carbon in soil was measured. Ground and unground labelled subclover leaves (Trifolium subterraneum) were added to a loamy sand and a cla...

Henry, O., 1989: The management of pleasure. Traditionally, access to and participation in cultural activities has been considered the perogative of the privileged few. However, the last decade has witnessed a sharp increase in the number of visits to heritage sites in France. In 1971 a stud...

Ravishankar, K. V.; Shaanker, R. Uma; Ganeshaiah, K. N., 1995: War of hormones over resource allocation to seeds: Strategies and counter-strategies of offspring and maternal parent. It is suggested that maternal parent and offspring have conflicting interests over the extent of resource allocation to developing seeds. While maternal parent would be selected to allocate her resources optimally among her offspring, the latter w...

Wesselinoff, W., 1942: S. anatum Infection in a Horse. A foal with colic was slaughtered. On examination no pathological changes were noted, but cultures from the kidney and liver showed, in addition to numerous coli-like organisms, some colonies of a rapidly growing organism which was found to have t...

de Visser, R.O.; Rissel, C.E.; Richters, J.; Smith, A.M.A., 2006: The impact of sexual coercion on psychological, physical, and sexual well-being in a representative sample of Australian women. It is well established that, compared to other women, women who have been forced or frightened into unwanted sexual activity have poorer psychological, physical, and sexual health. However, it is not clear whether particular experiences of sexual...

Zehr E.I.; Golden J.K., 1986: Strip and broadcast treatments of dichloropropene compared for controlling criconemella xenoplax and short life in a peach prunus persica orchard. A broadcast treatment of dichloropropene in soil before peach trees were planted was not more effective than a strip treatment (50% of surface area) for control of Criconemella xenoplax or improving tree growth, but the broadcast treatment was mor...

O'Shea, M.K.; Cane, P.A., 2004: Development of a highly sensitive semi-quantitative real-time PCR and molecular beacon probe assay for the detection of respiratory syncytial virus. Molecular beacons are a novel class of oligonucleotide probe capable of reporting the accumulation of target amplicon during real-time PCR by the emission of a fluorescent signal. A novel assay for the detection and estimation of respiratory syncy...

Pous J.; Munoz J. A.; Ledo J. J.; Liesa M., 1995: Partial melting of subducted continental lower crust in the Pyrenees. Journal of the Geological Society of London 152, Part 2: 217-220

Pal, A. K.; Das, S. N., 1990: Effect of growth regulators on growth and flowering of Lilium longiflorum. In an experiment carried out during 1989-90, NAA, GA3, IAA and MH at 100 or 200 p.p.m., or distilled water, were applied to potted plants of L. longiflorum as a foliar spray when the stems had reached a height of 5-6 cm. GA3, NAA and IAA applicati...