EurekaMag.com logo
+ Translate

Identification of a mutation in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs


, : Identification of a mutation in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs. Infection and Immunity 63(12): 4924-4927

We used transposon (TnphoA) mutagenesis to study the role of virulence factors of pathogenic Escherichia coli strains associated with septicemia in calves and piglets. We have produced an avirulent and serum-sensitive mutant of wild-type pathogenic strain 5131 O115:K"V165":F165 and have localized and identified the TnphoA insertion in the pstC gene of the pst-phoU operon. This operon encodes the PstSCAB transporter and PhoU protein that negatively regulate the phosphate (Pho) regulon. This mutation is pleiotropic and could have an effect on pathogenicity and on the production of the surface polysaccharides of strain 5131. The mutant demonstrated restored repressibility of alkaline phosphatase and regained the capacity to resist serum and to survive systemically for at least 5 days in experimentally inoculated pigs when complemented with plasmid pAN92, bearing the pst-phoU operon.

(PDF 0-2 workdays service)

Accession: 002863184

PMID: 7591158

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Daigle, F.F.irbrother, J.; Harel, J., 1995: Identification of a mutations in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs. We used transposon (TnphoA) mutagenesis to study the role of virulence factors of pathogenic Escherichia coli strains associated with septicemia in calves and piglets. We have produced an avirulent and serum-sensitive mutant of wild-type pathogeni...

Wang, X.; Cao, C.; Huan, H.; Zhang, L.; Mu, X.; Gao, Q.; Dong, X.; Gao, S.; Liu, X., 2016: Isolation, identification, and pathogenicity of O142 avian pathogenic Escherichia coli causing black proventriculus and septicemia in broiler breeders. Avian colibacillosis, characterized by black proventriculus and caused by avian pathogenic Escherichia coli (APEC) with an uncommon O142 serogroup, was diagnosed in young broiler breeders. Colonization and persistence assays performed in 7-day-old...

Daigle, F.; Forget, C.; Harel, J., 1996: Regulation of expression of F165-1 and F165-2 fimbriae in an Escherichia coli strain causing septicemia in pigs. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 162

Daigle, F.; Dozois, C.M.; Jacques, M.; Harel, J., 1997: Mutations in the f165(1)A and f165(1)E fimbrial genes and regulation of their expression in an Escherichia coli strain causing septicemia in pigs. Transposon (TnphoA) mutagenesis was used to study the expression of F165(1) fimbriae, related to Prs fimbriae, in the pathogenic Escherichia coli strain 5131 (O115:K "V165":F165). This strain causes septicemia in swine and also expresses...

Kariyawasam, S.; Johnson, T.J.; Nolan, L.K., 2005: The pap operon of avian pathogenic Escherichia coli strain O1:K1 is located on a novel pathogenicity island. We have identified a 56-kb pathogenicity island (PAI) in avian pathogenic Escherichia coli strain O1:K1 (APEC-O1). This PAI, termed PAI I[subscript APEC-O1], is integrated adjacent to the 3' end of the pheV tRNA gene. It carries putative viru...

Cheng, C.; Tennant, S.M.; Azzopardi, K.I.; Bennett-Wood, V.; Hartland, E.L.; Robins-Browne, R.M.; Tauschek, M., 2009: Contribution of the pst-phoU operon to cell adherence by atypical enteropathogenic Escherichia coli and virulence of Citrobacter rodentium. Strains of enteropathogenic Escherichia coli (EPEC) generally employ the adhesins bundle-forming pili (Bfp) and intimin to colonize the intestine. Atypical EPEC strains possess intimin but are negative for Bfp and, yet, are able to cause disease....

Ngeleka, M.; Jacques, M.; Martineau-Doizé, B.; Daigle, F.; Harel, J.; Fairbrother, J.M., 1993: Pathogenicity of an Escherichia coli O115:K"V165" mutant negative for F165(1) fimbriae in septicemia of gnotobiotic pigs. To evaluate the role of the F165(1) fimbrial system in the pathogenesis of septicemia, 2-day-old germfree pigs were inoculated intragastrically with Escherichia coli O115:K"V165":F165 wild-type strain 5131, its F165(1)-negative TnphoA mu...

Ngeleka, M.; Jacques, M.; Martineau Doize, B.; Daigle, F.; Harel, J.; Fairbrother, J.M., 1993: Pathogenicity of an Escherichia coli O115:KV165 mutant negative for F1651 fimbriae in septicemia of gnotobiotic pigs. To evaluate the role of the F165(1) fimbrial system in the pathogenesis of septicemia, 2-day-old germfree pigs were inoculated intragastrically with Escherichia coli O115:K"VI65":F165 wild-type strain 5131, its F165(1)-negative TnphoA mu...

Guarente, L.P.; Beckwth, J.; Wu, A.M.; Platt, T., 1979: A mutation distal to the messenger RNA endpoint reduces transcription termination in the tryptophan operon in Escherichia coli. Journal of Molecular Biology 133(1): 189-197

Gonzalez I.L.; Sheppard D.E., 1975: Genetic and physiologic characterization of revertants of a polar initiator mutation in the l arabinose operon of escherichia coli strain b r. Genetics 80(3 PART 1): S35-S36