EurekaMag.com logo
+ Translate

Identification of a nonpathogenic Erwinia amylovora guaB mutant


, : Identification of a nonpathogenic Erwinia amylovora guaB mutant. Plant Pathology (Oxford) 46(4): 594-599

A nonpathogenic Erwinia amylovora transposon mutant that has an insertion in the guaB gene was isolated. The mutation results in a nutritional requirement for guanine or xanthine, and loss of ability to produce ooze on immature pear fruit and to cause symptoms in the apple seedling assay. The mutant expressed other known virulence determinants including extracellular polysaccharide and had an intact hrp/dsp cluster. In addition it was able to grow in host tissue, although the population size in planta was maintained at a considerably lower level than that seen with the parent strain. The inability of the Erwinia amylovora guaB mutant to cause disease indicates that levels of guanine in plant tissue are likely to be insufficient to maintain optimal growth via the purine salvage pathway. This, in turn, appears to compromise the ability of the mutant to develop a sufficiently large population size in planta to overcome host defence mechanisms and cause disease symptoms. This indicates that a functional de novo guanine synthetic pathway is important for Erwinia amylovora to grow on plant tissue and cause disease.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863203

DOI: 10.1046/j.1365-3059.1997.d01-49.x

Download PDF Full Text: Identification of a nonpathogenic Erwinia amylovora guaB mutant


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Mane S.M.; Marzella L.; Bainton D.F.; Holt V.K.; Cha Y.; Hildreth J.E.K.; August J.T., 1989: Purification and characterization of human lysosomal membrane glycoproteins. Two human cell lysosomal membrane glycoproteins of .apprx. 120 kDa, hLAMP-1 and hLAMP-2, were identified by use of monoclonal antibodies prepared against U937 myelomonocytic leukemia cells or blood mononuclear cells. The two glycoproteins were pur...

Kim, J.; Song, J-Hwan.; Jeong, K-Yong.; Ee, H-Seok.; Seo, M-Kyo., 2015: Full three-dimensional power flow analysis of single-emitter-plasmonic-nanoantenna system. We present a full three-dimensional (3D) power flow analysis of an emitter-nanoantenna system. A conventional analysis, based on the total Poynting vector, calculates only the coupling strength in terms of the Purcell enhancement. For a better und...

Anonymous, 2004: Statement on intractable pain treatment acts. Journal of Pain & Palliative Care PharmacoTherapy 18(2): 93-98

Péter, I.; Szentirmay, Zán.; Schneider, Tás.; Radó, J.; Tóth, József., 2002: Angiocentric T-cell lymphoma. SUMMARY: The CD20+ variant of angiocentric T-cell lymphoma is an unusual type of T-cell lymphomas that present cystic changes in organs because of ischaemic necroses. The purpose of this study was to describe a case of CD20+angiocentric T-cell lym...

Reyes Audiffred, V.; Sotomayor Sanchez, S.M.; Gonzalez Juarez, L., 2007: Behaviors related to elderly population health in a developing community in Mexico City. Introduction: Aging is one of the more noticeable expressions of recorded changes in the worldwide demographic structure. The latest findings regarding determined factors of aging emphasize features related with healthy life style, resulting in sa...

Mayer, J., 1975: Scientific data processing in the determination of farming systems. International Crops Research Institute for the Semi Arid Tropics: India, ICRISAT: International workshop on farming systems November 18-21, 1974: 311-323

Khouri, I.; Keating, M.; Lerner, S.; O'brien, S.; Champlin, R., 1998: Improved survival with allogeneic and autologous stem cell transplantation for chronic lymphocytic leukemia A case-matched analysis with conventional chemotherapy. Blood 92(10 SUPPL 1 PART 1-2): 287A, Nov 15

Fernandez Lopez, S., 1984: Criterios elementales de reelaboracion tafonomica en ammonites de la Cordillera Iberica. Acta Geologica Hispanica, 192: 105-116

Camin, L.L., 1977: Comment on monitoring the Zr and 113Sn breakthrough in the 113mIn eluates from a 113Sn-113mIn generator. International Journal of Applied Radiation and Isotopes 28(12): 973-973

Kasparov, AK., 1986: Remains of mammals from the Late Paleolithic site Subhotino 4 in the Transbaikalia. Trudy Zoologicheskogo Instituta, 149: 98-106