+ Translate

Identification of a novel antigen from Corynebacterium pseudotuberculosis that protects sheep against caseous lymphadenitis

, : Identification of a novel antigen from Corynebacterium pseudotuberculosis that protects sheep against caseous lymphadenitis. Infection and Immunity 62(6): 2562-2567

A 40-kDa protein antigen from Corynebacterium pseudotuberculosis has been identified by application of a strategy that employs locally derived antibody-secreting cells (ASC). ASC probes generated by culture of ASC obtained from the lymph node draining the site of infection showed a specificity restricted to a 40-kDa antigen. Analysis of immunoblots with sequential serum samples taken from sheep during the course of experimental primary infection with C. pseudotuberculosis also revealed the 40-kDa antigen as an early immunodominant antigen. Sheep vaccinated with two 100-microgram doses of a 40-kDa antiagen preparation in aluminium hydroxide adjuvant were protected against infection with C. pseudotuberculosis, with an 82% reduction in the proportion of infected sheep and a 98% reduction in lung lesions. Sera from vaccinated sheep exhibited a strong response only to the 40-kDa antigen on immunoblots. These results strongly suggest that the 40-kDa antigen plays a major role in immunity to caseous lymphadenitis.

(PDF 0-2 workdays service)

Accession: 002863208

PMID: 8188379

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Ellis, J.A.; Hawk, D.A.; Mills, K.W.; Pratt, D.L., 1991: Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. Constituents of Corynebacterium pseudotuberculosis were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis of sonicated whole bacterial cells and ether-extracted cells revealed more than 35 bands in silver-...

Patel, A.R.; Chandel, B.S.; Chauhan, H.C.; Bulbule, N.R.; Bhalodia, S.D.; Patel, N.P., 2007: Corynebacterium pseudotuberculosis infection (Caseous lymphadenitis) in sheep and goats. Veterinary World 5(9): 299-302

Rebouças, M.F.; Portela, R.W.; Lima, D.D.; Loureiro, D.; Bastos, B.L.; Moura-Costa, L.F.; Vale, V.L.; Miyoshi, A.; Azevedo, V.; Meyer, R., 2011: Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats. In the current study, the applicability of the quantification of gamma interferon (IFN-γ) levels for the detection of animals infected with Corynebacterium pseudotuberculosis and for determining caseous lymphadenitis (CLA) clinical status was eva...

Ellis, J.A.; Hawk, D.A.; Holler, L.D.; Mills, K.W.; Pratt, D.L., 1990: Differential antibody responses to Corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis. Enzyme-linked immunosorbent assays (ELISA) with Corynebacterium pseudotuberculosis cell wall and bacteria-free supernatant with exotoxin preparations are antigens, and hemolysis inhibition tests were used to detect antibodies in the sera of adult...

LeaMaster, B.R.; Shen, D.T.; Gorham, J.R.; Leathers, C.W.; Wells, H.D., 1987: Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunologic protection of sheep against development of caseous lymphadenitis. The efficacy of a Corynebacterium pseudotuberculosis bacterin to protect sheep immunologically against development of caseous lymphadenitis was evaluated in controlled challenge-exposure experiments. Sixty-three mixed-breed, white-faced lambs were...

Jodor, N.; Haro, P.; Fernandez, A.; Gallardo, A.; Abalos, A.; Alvarez, L.; Diaz, Y.; Jensen, O.; Estevao Belchior, S., 2007: Caseous lymphadenitis: susceptibility to disinfectant in strains of Corynebacterium pseudotuberculosis, isolated from patagonian sheep. Caseous lymphadenitis (CLA) is a chronic, infectious, contagious disease caused by the bacterium Corynebacterium pseudotuberculosis. It results in abscesses of the lymph nodes in subcutaneous tissue as well as internal organs like lungs, liver and...

Zhao, H.K.; Morimura, H.; Hiramune, T.; Kikuchi, N.; Yanagawa, R.; Serikawa, S., 1991: Antimicrobial susceptibility of Corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in sheep in Hokkaido, Japan. Journal of Veterinary Medical Science 53(2): 355-356

Hodgson, A.L.; Tachedjian, M.; Corner, L.A.; Radford, A.J., 1994: Protection of sheep against caseous lymphadenitis by use of a single oral dose of live recombinant Corynebacterium pseudotuberculosis. An inactive form of the Corynebacterium pseudotuberculosis phospholipase D (PLD) gene was constructed and expressed in a PLD-negative strain (designated Toxminus) of C. pseudotuberculosis. Antibody responses specific to Toxminus and both Toxminus...

Muckle, C.; Gyles, C., 1983: Relation of lipid content and exotoxin production to virulence of Corynebacterium pseudotuberculosis in mice Agent of caseous lymphadenitis of sheep and goats. American journal of veterinary research 44(6): 1149-1153

Doty, R.B.; Dunne, H.W.; Hokanson, J.F.; Reid, J.J., 1964: A Comparison Of Toxins Produced By Various Isolates Of Corynebacterium Pseudotuberculosis And The Development Of A Diagnostic Skin Test For Caseous Lymphadenitis Of Sheep And Goats. All of 121 cultures of Corynebacterium pseudotuberculosis isolated from 105 sheep, 9 goats, 6 horses, and 1 ox produced soluble exotoxin in suitable liquid mediums. Specific antitoxin was produced in rabbits against the toxins' of isolates fr...