EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of a potexvirus in Korean garlic plants


, : Identification of a potexvirus in Korean garlic plants. Agricultural Chemistry & Biotechnology 38(1): 55-62

To understand the molecular structure of Korean garlic viruses, cDNA cloning of virus genomic RNA was attempted. Virus particles were isolated from virus-infected garlic leaves and a cDNA library was constructed from garlic virus RNA. One of these clones, S81, selected by random sequencing has been identified as a member of potexvirus group other than potyvirus and carlavirus. The clone is 873 hp long contains most of the coat protein (CP) coding region and 3'-noncoding region including poly(A) tail. A putative polyadenylation signal sequence (AAUAAA) and the hexanucleotide motif (ACUUAA), a replicational cis-acting element conserved in the 3'-noncoding region of potexvirus RNAs are noticed. The clone S81 shows about 30-40% identity in both nucleotide and amino acid sequences with CPs of potexviruses. The genome size of the virus was analyzed to be 7.46 knt by Northern blot analysis, which was longer than those of other potexviruses. The open reading frame encoding CP was expressed as a fusion chromatography. Polyclonal antibody was raised against S81CP in rabbit to examine the occurrence of garlic potexvirus in Korean garlic plants by immunoblot analysis. Two virus protein bands of Mr 27,000 and 29,000 from garlic leaf extract of various cultivars reacted with the antibody. It was shown that Mr 27,000 band might not be a degradation product of Mr 29,000 band, suggesting that two types of potexvirus different in size of coat protein could exist in Korean garlic plants.


Accession: 002863223

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Miglino, R.; Jodlowska, A.; Schadewijk, A.R. van, 2006: Detection and identification of a novel Potexvirus infecting Allium by paramagnetic beads ssRNA isolation and one tube RT-PCR assay with a new Potexvirus genus primer set. A rapid paramagnetic capture/reverse transcription PCR assay procedure was developed for the detection of viruses from the genus Potexvirus. The Potexvirus ssRNA was selectively isolated from plant sap by streptavidin coated paramagnetic beads cou...

Song SangIk; Song JongTae; Chang MooUng; Lee JongSeob; Choi YangDo, 1997: Identification of one of the major viruses infecting garlic plants, garlic virus X. A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic...

Song, S.I.; Song, J.T.; Chang, M.U.; Lee, J.S.; Choi, Y.D., 1998: Identification of one of the major viruses infecting garlic plants, garlic virus X. A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic...

Moreno P.; Attathom S.; Weathers L.G., 1976: Identification transmission and partial purification of a potexvirus causing a disease of nandina plants in california usa. Proceedings of the American Phytopathological Society 3: (1977)

Ahn, J-Jun.; Akram, K.; Lee, J.; Kim, K-Su.; Kwon, J-Ho., 2012: Identification of a gamma-irradiated ingredient (garlic powder) in Korean barbeque sauce by thermoluminescence analysis. Thermoluminescence (TL) analysis was applied to identify gamma-irradiated garlic powder in Korean barbeque sauce before and after pasteurization (85 C, 3 min), when blended in different ratios (1%, 3%, and 5%). The sauce sample with nonirradiated...

Lee, W.S., 1973: Physiological and ecological studies on Korean local strains of garlic. 1. On the process of sprouting in stored garlic. In studies on 33 local garlic cvs collected from all parts of Korea, the time at which sprouting occurred in storage was very different in early and late cvs. In early cvs leaf elongation began in early August, and in late August or early Septembe...

Iwasaki, M.; Ishihara, A., 1977: Studies on a lifting type digger for root crops. 1. Forms and pulling resistance of baker's garlic and garlic plants. In mechanical harvesting studies, the percentage of Baker's garlic plants which could be pulled without the cutting of leaves was highest (54-100%) at the end of May. Multiple regression analyses of the max. pulling resistance showed that bul...

Lee, Y.S.; Hsu, Y.H.; Lin, N.S., 2000: Generation of subgenomic RNA directed by a satellite RNA associated with bamboo mosaic potexvirus: analyses of potexvirus subgenomic RNA promoter. Satellite RNA of bamboo mosaic potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic potexvirus (BaMV) for replication and encapsidation. A full-length cDNA clone of s...

Yanazawa, T.; Fujii, S., 1972: Studies on leaf chlorosis in Baker's garlic grown on sandy soils. IV. Foliar sprays for correcting zinc deficiencies in garlic plants. Foliar sprays of ZnSO4, ZnCl2 and Zn-EDTA were markedly and similarly effective in reducing chlorosis and encouraged normal growth and good yields; early applications corrected the symptoms most rapidly. The treatment recommended is to spray 3 tim...

Kim, B.W.; Lee, B.Y.; Moon, W.; Pyo, H.K., 1979: Study on growth and bulb formation in garlic plants (Allium sativum L.). II. The effect of night interruption for various periods with different light qualities on growth and bulb formation in 6-cloved garlic plants. The long-day conditions necessary [see the preceding abstract] for growth and bulb formation in garlic plants could be replaced by night interruption with incandescent, red or far-red light. The interruption was most effective when applied in the...