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Identification of a silencer module which selectively represses cyclic AMP-responsive element-dependent gene expression


, : Identification of a silencer module which selectively represses cyclic AMP-responsive element-dependent gene expression. Molecular and Cellular Biology 15(11): 6139-6149

The cyclic AMP (cAMP)-inducible promoter from the rat lactate dehydrogenase A subunit gene (LDH A) is associated with a distal negative regulatory element (LDH-NRE) that represses inherent basal and cAMP-inducible promoter activity. The element is of dyad symmetry, consisting of a palindromic sequence with two half-sites, 5'-TCTTG-3'. It represses the expression of an LDH A/chloramphenicol acetyltransferase (CAT) reporter gene in a dose-dependent, orientation- and position-independent fashion, suggesting that it is a true silencer element. Uniquely, it selectively represses cAMP-responsive element (CRE)-dependent transcription but has no effect on promoters lacking a CRE sequence. The repressing action of LDH-NRE could be overcome by cotransfection with LDH A/CAT vector oligonucleotides containing either the LDH-NRE or CRE sequence. This suggests that the reversal of repression was caused by the removal of functional active, limiting transacting factors which associate with LDH-NRE as well as with CRE. Gel mobility shift, footprinting, and Southwestern blotting assays demonstrated the presence of a 69-kDa protein with specific binding activity for LDH-NRE. Additionally, gel supershift assays with anti-CREB and anti-Fos antibodies indicate the presence of CREB and Fos or antigenically closely related proteins with the LDH-NRE/protein complex. We suggest that the LDH-NRE and CRE modules functionally interact to achieve negative modulation of cAMP-responsive LDH A transcriptional activity.

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Accession: 002863242

PMID: 7565766

DOI: 10.1128/mcb.15.11.6139

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Xu, Mei; Zhang; Xue; Jin; Chunlian, 1994: The detection of the amplification of the myc protooncogenes in non-small cell lung cancer by polymerase chain reaction. We detected the amplification of the myc protooncogenes in fresh human tumor tissues from 33 patients with non-small cell lung cancers, using polymease chain reaction with single primer set. Five tumors were revealed to have one amplified myc gene...

Julia, V.; Su, X.; Gebhart, G. F., 1997: Effect of a NK3 receptor antagonist on mechanosensitive pelvic nerve afferent fibers in rats. Society for Neuroscience Abstracts 23(1-2): 1261

Fed Amer Soc Exp Biol, 1970: Part on regulation of protein synthesis april 16 1970. Federation Proceedings 29(2): 735-736

Mok, J.; Pembrey, L.; Tovo, P-A.; Newell, M-L., 2005: When does mother to child transmission of hepatitis C virus occur?. Objective: To investigate when hepatitis C virus (HCV) infection from mother to child occurs, and evaluate possible associated factors. Design: Prospective cohort study. Patients: Fifty four HCV infected children tested within three days of birth...

Doi Y.; Yonezawa Y.; Seo H.; Yamada M.; Takata J.; Chikamori T.; Yamasaki F.; Ozawa T.; Kuzume O.; Hamashige N., 1991: Prognosis in medically treated ischemic heart disease. Japanese Circulation Journal 55(SUPPL A): 112

Emlen, D. J., 2003: Endocrine regulation of beetle horn expression Insights for horn evolution. SICB Annual Meeting & Exhibition Final Program & Abstracts : 165

Levine, A.E.; Walsh, K.A., 1980: Purification of an acrosin-like enzyme from sea urchin sperm. Sea urchin sperm contain an acrosin-like enzyme with an apparent molecular weight of 53,000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals two subunits of 34,000 daltons and 18,000 daltons. The Mr = 34,000 subunit is the cataly...

Bebee, Cn, 1986: Nuclear fallout effects on agriculture, 1970-March 1986. Quick bibliography series National Agricultural Library US: 86 (86-77)

Bostrom, Kristina; Watson, Karol E.; Stanford, William P.; Ravindranath, Rajeswari M. H.; Demer, Linda L., 1994: Calcification of aortic medial cells is regulated by bone morphogenetic protein and surface gangliosides in vitro. Circulation 90(4 PART 2): I290

Sreenivasaprasad, S.; Mills, P. R.; Brown, A. E., 1993: Adaption of automated microbiology identification system (AMBIS) to determine fungal relationships using DNA polymorphisms. The beta scanner and pattern matching software integrated in the automated microbiology identification system (AMBIS) were used to assess the relatedness of isolates of Colletotrichum acutatum, C. kahawae, C. fragariae [Glomerella cingulata], C. g...