EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of a silencer module which selectively represses cyclic AMP-responsive element-dependent gene expression


, : Identification of a silencer module which selectively represses cyclic AMP-responsive element-dependent gene expression. Molecular and Cellular Biology 15(11): 6139-6149

The cyclic AMP (cAMP)-inducible promoter from the rat lactate dehydrogenase A subunit gene (LDH A) is associated with a distal negative regulatory element (LDH-NRE) that represses inherent basal and cAMP-inducible promoter activity. The element is of dyad symmetry, consisting of a palindromic sequence with two half-sites, 5'-TCTTG-3'. It represses the expression of an LDH A/chloramphenicol acetyltransferase (CAT) reporter gene in a dose-dependent, orientation- and position-independent fashion, suggesting that it is a true silencer element. Uniquely, it selectively represses cAMP-responsive element (CRE)-dependent transcription but has no effect on promoters lacking a CRE sequence. The repressing action of LDH-NRE could be overcome by cotransfection with LDH A/CAT vector oligonucleotides containing either the LDH-NRE or CRE sequence. This suggests that the reversal of repression was caused by the removal of functional active, limiting transacting factors which associate with LDH-NRE as well as with CRE. Gel mobility shift, footprinting, and Southwestern blotting assays demonstrated the presence of a 69-kDa protein with specific binding activity for LDH-NRE. Additionally, gel supershift assays with anti-CREB and anti-Fos antibodies indicate the presence of CREB and Fos or antigenically closely related proteins with the LDH-NRE/protein complex. We suggest that the LDH-NRE and CRE modules functionally interact to achieve negative modulation of cAMP-responsive LDH A transcriptional activity.


Accession: 002863242

PMID: 7565766

DOI: 10.1128/mcb.15.11.6139

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Bernath V.A.; Muro A.F.; Vitullo A.D.; Bley M.A.; Baranao J.L.; Kornblihtt A.R., 1990: Cyclic amp inhibits fibronectin gene expression in newly developed granulosa cell line by a mechanism that suppresses cyclic amp responsive element dependent transcriptional activation. The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several...

Nagamoto-Combs, K.; Piech, K.M.; Best, J.A.; Sun, B.; Tank, A.W., 1997: Tyrosine hydroxylase gene promoter activity is regulated by both cyclic AMP-responsive element and AP1 sites following calcium influx. Evidence for cyclic amp-responsive element binding protein-independent regulation. Membrane depolarization of PC 12 cells using 50 mM KCl leads to induction of tyrosine hydroxylase (TH) mRNA. This induction of TH mRNA is apparently due to increased TH gene promoter activity mediated by the influx of Ca-2+. In PC 12 cells transie...

Kirov, N.; Childs, S.; O'Connor, M.; Rushlow, C., 1994: The Drosophila dorsal morphogen represses the tolloid gene by interacting with a silencer element. The dorsal protein (DL) regulates the transcriptional activity of several genes that determine cell fate along the dorsoventral axis of the Drosophila melanogaster embryo. DL is present at high levels in ventral nuclei, where it activates some gen...

Gaunitz, F.; Heise, K.; Gebhardt, R., 2003: A silencer element in the first intron of the glutamine synthetase gene represses induction by glucocorticoids. The enzyme glutamine synthetase (GS) ranks as one of the most remarkable glucocorticoid-inducible mammalian genes. In many tissues and cell lines, the synthetic glucocorticoid dexamethasone alone increases GS expression several fold. The direct re...

Hussain, S.; Kilbey, A.; Gillespie, D.A., 1998: v-Jun represses c-jun proto-oncogene expression in vivo through a 12-O-tetradecanoylphorbol-13-acetate-responsive element in the proximal gene promoter. c-jun proto-oncogene expression is extinguished in cells transformed by v-Jun; however, the mechanistic basis of this phenomenon has not been elucidated. c-jun mRNA levels are greatly reduced in v-Jun-transformed cells, and we show that this reduc...

Morales, V.; Gonzalez-Robayna, I.; Santana, M.Pino.; Hernandez, I.; Fanjul, L.F., 2006: Tumor necrosis factor-alpha activates transcription of inducible repressor form of 3',5'-cyclic adenosine 5'-monophosphate-responsive element binding modulator and represses P450 aromatase and inhibin alpha-subunit expression in rat ovarian granulosa cells by a p44/42 mitogen-activated protein kinase-dependent mechanism. The proinflammatory cytokine TNFalpha has important actions at the level of the ovary, including inhibition of P450 aromatase (P450AROM) activity and the secretion of inhibin, two proteins that are markers of the granulosa cell's differentiat...

Morales, V.; Gonzalez-Robayna, I.; Santana, M.P.no; Hernandez, I.; Fanjul, L., F., 2006: Tumor necrosis factor-alpha activates transcription of inducible repressor form of 3 ', 5 '-cyclic adenosine 5 '-monophosphate-responsive element binding modulator and represses p450 aromatase and inhibin alpha-subunit expression in rat ovarian granulosa cells by a p44/42 mitogen-activated protein kinase-dependent mechanism. The proinflammatory cytokine TNF alpha has important actions at the level of the ovary, including inhibition of P450 aromatase (P450(AROM)) activity and the secretion of inhibin, two proteins that are markers of the granulosa cell's different...

Bernath, V.A.; Muro, A.F.; Vitullo, A.D.; Bley, M.A.; Barañao, J.L.; Kornblihtt, A.R., 1990: Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation. The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several...

Mizoi, J.; Ohori, T.; Moriwaki, T.; Kidokoro, S.; Todaka, D.; Maruyama, K.; Kusakabe, K.; Osakabe, Y.; Shinozaki, K.; Yamaguchi-Shinozaki, K., 2013: GmDREB2A;2, a canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-type transcription factor in soybean, is posttranslationally regulated and mediates dehydration-responsive element-dependent gene expression. Soybean (<I>Glycine max</I>) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2)...

Lund J.; Ahlgren R.; W.D.; Kagimoto M.; Simpson E.R.; Waterman M.R., 1990: Transcriptional regulation of the bovine cyp17 p 450 17 alpha gene identification of two cyclic amp regulatory regions lacking the consensus cyclic amp responsive element cre. Regions within the 5'-flanking sequence of the bovine CYP17 (P-45017.alpha.) gene which are required for cAMP-dependent regulation of transcription have been localized by transient transfection of chimeric reporter gene constructs into mouse...