EurekaMag.com logo
+ Translate

Identification of a viroid associated with fruit disorder of Japanese pear


, : Identification of a viroid associated with fruit disorder of Japanese pear. Bulletin of the Fruit Tree Research Station 0(27): 65-77

Recently, abnormal fruit disorders of Japanese pear cultivars, Niitaka and Yoshino, have been observed in Chiba, Ibaraki and Oita Prefectures. The characteristic symptoms consist of a rough fruit shape. In order to determine whether viroids are the causal agents of this disorder, RNA was isolated from Japanese pear with the disorder. Electrophoresis on polyacrylamide gels showed two bands (RNA1, RNA2) which were not detected in extracts from healthy trees. RNA1 was susceptible to RNaseA in 0.1 times SSC (0.15M NaCl, 0.015M sodium citrate) and 2.0 times SSC. RNA2 was susceptible to RNaseA in 0.1 times SSC but was resistant to RNaseA in 2.0 times SSC. These reactions to RNaseA indicated that RNA1 displayed a single-stranded structure and RNA2 was double-stranded (ds) RNA. The mobility of RNA1 and RNA2 on PAGE was similar to that of apple scar skin viroid (ASSVd) and apple scar skin associated RNA2 (ASSARNA2), respectively. RNA1 was detected by return, bidirectional and two-dimensional PAGE. RNA1 purified by electrophoresis was transmitted to pear and apple seedlings by the carborundum or razor-slash method and multiplied in them. The 32P-labeled ASSVd cDNA probe hybridized with RNA1. Healthy apple trees of cv. Starking Delicious, crab apple NY11894 were inoculated by grafting with Japanese pear's bark or bud with the fruit disorder. Dappling and scaring appeared in cv. Starking Delicious fruits. Dappling and size reduction appeared in NY11894 fruits. These results indicated that RNA1 is a viroid similar to ASSVd. Our results suggest that this viroid is strongly associated with the Japanese pear fruit disorder. However it remains to be determined whether this viroid is the causal agent of Japanese pear fruit disorder.

(PDF 0-2 workdays service)

Accession: 002863262

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Fekih-Hassen, I.R.ussel, S.K.mmert, J.F.khfakh, H.M.rrakchi, M.J.jakli, M., 2006: Development of a Rapid RT-PCR Test for the Detection of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid and Apple Scar Skin Viroid in Fruit Trees from Tunisia. A reverse transcription polymerase chain reaction was developed to investigate the occurrence of 'peach latent mosaic viroid' (PLMVd), 'pear blister canker viroid' (PBCVd), 'hop stunt viroid' (HSVd) and 'apple sc...

Hassen, I.F.; Kummert, J.; Marbot, S.; Fakhfakh, H.; Marrakchi, M.; Jijakli, M.H., 2004: First report of Pear blister canker viroid, Peach latent mosaic viroid, and Hop stunt viroid infecting fruit trees in Tunisia. A survey of fruit trees was carried out in 17 orchards in the northern and Sahel regions of Tunisia. Samples were collected in field trees of peach (Prunus persica), pear (Pyrus communis) and almond (Prunus dulcis) that showed symptoms potentially...

Osaki, H.; Kudo, A.; Ohtsu, Y., 1996: Japanese pear fruit dimple disease caused by apple scar skin viroid (ASSVd). An abnormal fruit disorder of Japanese pear cvs. Niitaka and Yoshino, had symptoms of dimpling of the mature fruit surface. A viroid was detected in association with this disorder. Healthy Niitaka and Yoshino trees were back-inoculated with the vi...

Takatsuji, T.; Aoba, K., 1983: Studies on the fruit hardening disorder of japanese pear 1. fruit characteristics water relations and root activities of disordered trees. Fruit hardness, alcohol insoluble substance (AIS) and K concentrations were higher and Ca concentration or Ca/K content ratio was lower in affected fruits as compared with normal fruits. AIS concentrations in several parts of the fruit were correl...

Takatsuji, T.; Aoba, K., 1984: Studies on the fruit hardening disorder of Japanese pear. I. Fruit characteristics, water relations and root activities of disordered trees. In studies of 4-6 different cultivars, those which showed no symptoms or only slight symptoms of the disorder had higher osmotic potential and higher fruit sugar contents than the others. There were negative correlations between both fruit sugar c...

Kawamata, S., 1979: Studies on a hardening disorder of Japanese pear (Pyrus serotina var. culta Rehder) fruit. II. Effects of calcium supply and soil moisture on the incidence of the yuzuhada disorder in pot-grown Nijisseiki pears. At a soil moisture status of pF 2.2 the incidence of the yuzuhada disorder was progressively reduced by annual applications of CaO2 or CaO. At pF 2.6 and 1.3, however, Ca application reduced the disorder in the first year of application only and t...

Umemoto, S., 1992: Seasonal changes in the susceptibility of japanese pear pyrus serotina rehd. cv. kosui fruit to the japanese pear scab fungus venturia nashicola. The effects of the fruit growth stage on the susceptiblity to and the latent period of scab fungus on Japanese pear cv. "Kosui" were investigated. The degree of fruit susceptibility to the scab disease was high from the fruit receptacle...

Takatsuji, T.; Aoba, K., 1985: Studies on the fruit hardening disorder of Japanese pear. II. Ca nutritional characteristics of affected trees. The movement of Ca into fruits from other parts of the tree appeared to be reduced in affected trees. 45Ca fed from a solution for 40 days during the growing season was mostly distributed in actively growing parts of the tree such as young shoots...

Zhang,C.; Tanabe,K.; Mori,M.; Tani,H.; Nakajima,H.; Wang,S.; Choi,J.H., 2008: Identification and quantification of bioactive gibberellins by GC-MS in Japanese pear fruit. To clarify the role of gibberellins (GAs) in fruit development in Japanese pear (cultivars Atago and Shinkou), biologically active GAs in fruits were identified and quantified by GC-MS. Besides GA<sub>3</sub>, GA<sub>1</sub>...

Takatsuji, T.; Aoba, K., 1985: Studies on the fruit hardening disorder of japanese pear 2. calcium nutritional characteristics of disordered trees. The relationships between the Ca nutrition of fruits and the incidence of fruit hardening were studied. There were significant negative correlations between the ratio of disordered fruits and the ratio of fruit Ca to leaf Ca content. Movement of C...