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Identification of an early marker of embryogenesis in pine


, : Identification of an early marker of embryogenesis in pine. Somatic cell genetics and molecular genetics of trees: 29-38

In order to characterize cell wall proteins specific for early somatic embryo development in Carribean pine (Pinus caribaea), 3 glycosylated polypeptides representative of all embryogenic lines were chosen from the polypeptides ionically bound to cell surfaces. N-terminal amino acid sequencing of one polypeptide revealed homologies with sequences of germin precursors deduced from cDNA sequencing in other plant species and with mature germins of wheat and barley.

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BULLIVANT, S., 1965: Freeze Substitution And Supporting Techniques. Tissue samples of the size normally used in electron microscopy (of the order of 1 mm. on edge) can be directly frozen and preserved without ice crystal formation. In the pancreas, glycerination followed by freezing in liquid propane, substitution...