+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of antigens of pathogenic free-living amoebae by protein immunoblotting with rabbit immune and human sera

Identification of antigens of pathogenic free-living amoebae by protein immunoblotting with rabbit immune and human sera

Clinical and Diagnostic Laboratory Immunology 1(5): 493-499

Prominent antigens of pathogenic and nonpathogenic free-living amoebae were identified by using polyclonal rabbit immune sera in immunoblot assays. The intent was to determine if prominent epitopes identified with rabbit immune sera could also be recognized by human sera. With rabbit sera, the development of immunoreactive bands was restricted to molecular masses of greater than 18.5 kDa for Naegleria, Hartmannella, and Vahlkampfia antigens. Two or more broad bands of less than 18.5 kDa were prominent features in three different Acanthamoeba species. Few cross-reactive antibodies could be detected between representative species of the three different subgroups of Acanthamoeba. Naegleria antigen was likewise serologically distinct, as were Hartmannella and Vahlkampfia antigens. The relative lack of cross-reacting antibodies between the pathogenic amoebae suggested that it would be desirable to use a panel of amoebic antigens to represent the range of serologically distinct antigens for assessing reactive antibodies in human sera. In pooled human serum (10 serum specimens per pool), the appearance of minimally reactive bands ranging from 32.5 to 106 kDa was a common feature of all six antigens. A prominent band of less than 18.5 kDa was identified in the Acanthamoeba culbertsoni antigen lane in 2 of the 10 human serum specimen pools. When the sera from each of the two groups were tested individually by immunoblotting, the reaction with the A. culbertsoni antigen could be associated with one individual. By using a panel of amoebic antigens, this method could prove useful in recognizing undiagnosed amoebic infections by revealing specific reactive antibodies.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 002863322

Download citation: RISBibTeXText

PMID: 8556491

Related references

Virulence of free living soil amoebae and identification of pathogenic naegleria hartmannella types of amoebae which cause meningo encephalitis in men and women. Journal of Protozoology 18(Suppl.): 43, 1971

Isolation, axenization and protein content of pathogenic and non-pathogenic small free-living amoebae. Biological Memoirs 25(1): 5-8, 1999

In vitro effect of drugs against pathogenic and non-pathogenic free-living amoebae and on anaerobic amoebae. Indian Journal of Experimental Biology 10(1): 43-45, 1972

Pathogenic free-living amoebae in the human throat. Revista Iberica de Parasitologia 49(2): 125-126, 1989

Isolation and identification of Giardia cysts, Cryptosporidium oocysts and free living pathogenic amoebae in water etc, 1989. Unknown, 1990

Isolation and identification of potentially pathogenic free-living amoebae in samples from environments in a public hospital in the city of Porto Alegre, Rio Grande do Sul. Revista Da Sociedade Brasileira de Medicina Tropical 40(3): 316-320, 2007

Identification of Entamoeba histolytica antigens recognized by IgA class human antibodies in sera and colostra of puerperal women using immunoblotting techniques. Archivos de Investigacion Medica 21(Suppl. 1): 97, 1990

Comparative investigations on metabolism of pathogenic and non pathogenic strains of free living amoebae. Acta Protozoologica 26(1): 45-58, 1987

An investigation of the pathogenic and non-pathogenic free-living amoebae in an activated-sludge plant. Water Air & Soil Pollution. 69(1-2): 135-139, 1993

Concanavalin A induced agglutination of pathogenic and non-pathogenic small free-living amoebae. Indian Journal of Parasitology 6(1): 43-49, 1982

Occurrence of pathogenic and non pathogenic free-living amoebae in swimming pools and thermal mud in an Italian spa. Giornale di Malattie Infettive e Parassitarie 36(4): 282-287, 1984

First report of free living pathogenic and non pathogenic amoebae in an aquatic vegetable, Trapa bispinosa Singhada. 2012

Control of legionella pneumophila pathogenic free living amoebae and pathogenic fungi in cooling waters. Jolley, R L , W A Brungs And R B Cumming (Ed ) Water Chlorination: Environmental Impact And Health Effects, Vol 3 ; 3rd Conference, Colorado Springs, Colo , Usa, Oct 28-Nov 2, 1979 Xxviii+1171p Ann Arbor Science Publishers, Inc : Ann Arbor, Mich , Usa Illus P29-38, 1980

An investigation of the pathogenic and non-pathogenic free-living amoebae from the root zone method of wastewater treatment. Water Air & Soil Pollution. 69(1-2): 93-98, 1993

Meningo encephalitis due to pathogenic free living amoebae report of 2 cases human naegleria gruberi acanthamoeba astronyxis. Journal Of The American Medical Association: 579-582, 1968