+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of bovine viral diarrhea virus receptor in different cell types

, : Identification of bovine viral diarrhea virus receptor in different cell types. Veterinary Microbiology 49(1-2): 67-79

Anti-idiotypic antibodies (anti-ids) have been used successfully in studies on bovine viral diarrhea virus (BVDV) receptor(s) in our laboratory. The anti-ids specifically bound to cultured cells and identified a 50 kDa cellular membrane protein, which is thought to be a specific receptor for BVDV. In this study, flow cytometric analyses demonstrated that the anti-ids also specifically bound to different cell types, namely MDBK, EBK, BT, PK15, MA104, and Vero. Experiments on virus attachment and replication showed that BVDV adsorbed to all cells and replicated in them except monkey kidney cells MA104 and Vero (non-permissive). Results from plaque reduction assays indicated that cellular membrane proteins from all cell lines competitively inhibited BVDV attachment to cultured MDBK cells, suggesting the presence of BVDV receptor on all cells. Immunoblotting of cell membrane proteins with the anti-ids revealed a 50 kDa protein in both permissive and nonpermissive cells. Subcloned or synchronized MDBK cells demonstrated no significant difference of binding with anti-ids as compared to normal cultured cells.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863348

PMID: 8861644

DOI: 10.1016/0378-1135(95)00167-0

PDF Full Text: Identification of bovine viral diarrhea virus receptor in different cell types

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Fritzlar, Frank., 2004: Three new leafbeetle species Coleoptera, Chrysomelidae, Alticinae from southwestern Turkey Aphthona alanyensis n sp, Aphthona bergealoides n sp und Longitarsus manfredi n sp - drei Blattkaefer Coleoptera, Chrysomehdae, Alticinae aus der suedwestlichen Tuerkei. Three leaf-beetle species are described from the Antalya and Icel (Mersin) districts in southern Turkey: Aphthona alanyensis n. sp., A. bergealoides n. sp., and L. manfredi n. sp. Distinguishing characters of the new species and similar congeners...

Goes, R.N.; Simons, A.J.; Beart, R.W., 1996: Level of highest mean resting pressure segment in the anal canal. A quantitative assessment of anal sphincter function. Even with the development of new technologies, the mechanism of fecal continence is still not completely understood. This study evaluates the relative position of the highest mean resting pressure (HMRP) in the anal canal and its correlation with...

NEWTON, K., 2012: The nurse in poliomyelitis care. American Journal of Nursing 47(6): 370-372

Girgis, F.G., 1967: A study on the early fate of acrtilage homograft in the rat. Journal of the Egyptian Medical Association 50(3): 212-220

Davis, M.B.con, R.; Beyrouty, C., 1991: Alumni opinions on recruitment and curriculum in agronomy. Journal of agronomic educationng 20(1): 40-42

Nord E.C.; Hartless P.F.; Nettleton W.D., 1971: Effects of several factors on saltbush d establishment in california. Journal of Range Management 24(3): 216-223

Edery-Halpern, G.; Nachson, I., 2004: Distinctiveness in flashbulb memory: comparative analysis of five terrorist attacks. The purpose of the present study was to find out whether a series of terrorist attacks, which share some common features, elicit flashbulb memories (of the personal circumstances in which the person first learned about these events) that are usual...

Zhang, C.; Zhang, Q.; Chen, C.; Jiang, N.; Liu, D.; Qiu, K.; Liu, S.; Wu, B., 2013: Metro-access integrated network based on optical OFDMA with dynamic sub-carrier allocation and power distribution. We propose and demonstrate a novel optical orthogonal frequency-division multiple access (OFDMA)-based metro-access integrated network with dynamic resource allocation. It consists of a single fiber OFDMA ring and many single fiber OFDMA trees, wh...

Rosenberger, Andreas, 2009: Arrival-time order location revisited. A fast method to estimate an earthquake epicentral region from the very first P-wave arrivals is relevant in the context of early warning and rapid response systems. Arrival-time order location (AOL) uses the sequence in which an array of sensors...

Slezak, R.; Luczak, K.; Kalscheuer, V.; Neumann, T.E.; Sasiadek, M.M., 2010: Noonan-like/multiple giant cell lesion syndrome in two adult patients with SOS1 gene mutations. Clinical Dysmorphology 19(3): 157-160