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Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells

, : Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells. Planta 02(3): 341-348

Oligogalacturonide-induced modifications of protein phosphorylation in cells of Nicotiana tabacum L. were investigated by in-vitro phosphorylation of plasma-membrane-enriched fractions and electrophoretic analysis on two-dimensional gels. About 100 polypeptides were resolved; among these 40 phosphoproteins were detected and their 33P-labelling quantified. Most of the phosphorylations were inhibited by staurosporine and several proteins were hyperphosphorylated in the presence of okadaic acid, indicating the presence of protein phosphatase(s) in addition to staurosporine-susceptible protein kinase(s) in the plasma-membrane-enriched fraction. In the presence of oligogalacturonides, phosphorylation of seven acidic polypeptides ranging from 15 to 65 kDa was strongly enhanced. A twofold enhancement of the phosphorylation of a 24-kDa protein and a two- to threefold decrease in the phosphorylation of acidic proteins of Mrs 62, 65, 80 and 84 was also observed in response to oligogalacturonides. One of the oligogalacturonide-modulated phosphoproteins was identified as calreticulin by direct nucleotide sequencing after preparative two-dimensional electrophoresis and comparison with protein database sequences. Decreased phosphorylation of calreticulin was also observed in vivo, shortly after addition of oligogalacturonides to tobacco cells, confirming the biological relevance of the modification. Although the presence of calreticulin, an abundent reticuloplasmin with high calcium-binding capacity, has been reported in both mammalian and plant cells its function is as yet largely unknown. Modulation of the phosphorylation of a plant calreticulin-like protein by oligogalacturonides is shown here, suggesting a role in the early transduction steps of this signal.

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Accession: 002863356

PMID: 9232905

DOI: 10.1007/s004250050136

PDF Full Text: Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells

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Cameron, H.U., 1991: Failure of a titanium endoprosthesis--a case report. A titanium-head Thomson endoprosthesis was revised 2.5 years after insertion. Metallosis with burnishing of the head and acetabular erosion were noted. The stem and the cement mantle were in perfect condition. Because this is not an isolated case,...

Maclennan D.H.; Klip A.; Reithmeier R.; Michalak M.; Campbell K.P., 1980: Possible sites of ion flow in the sarcoplasmic reticulum membrane. Lee, C P , G Schatz And L Ernster (Ed ) Membrane Bioenergetics; Based on The International Workshop; Bloomfield Hills, Mich , Usa, July 5-7, 1979 Xxxiv+609p Addison-Wesley Publishing Co , Inc : Reading, Mass , Usa Illus Paper P255-266, 1979

Zitvogel, L.; Robbins, P.D.; Storkus, W.J.; Clarke, M.R.; Maeurer, M.J.; Campbell, R.L.; Davis, C.G.; Tahara, H.; Schreiber, R.D.; Lotze, M.T., 1996: Interleukin-12 and B7.1 co-stimulation cooperate in the induction of effective antitumor immunity and therapy of established tumors. Interleukin-12 (IL-12) promotes specific and long-lasting anti-tumor immunity mediated by T cells in a variety of murine tumor models. IL-12 also synergizes with B7.1 (CD80) co-stimulation to induce proliferation and cytokine production by both hu...

Castrillo-Bustamante, C.; Vejo-Puente, E.; Velasco-Montes, J.; Hernández-Hernández, Jé.Luis., 2008: Severe hypokalemia and tenofovir. Enfermedades Infecciosas Y Microbiologia Clinica 26(5): 317-318

Maher, E.; Creane, A.; Sultan, S.; Hynes, N.; Lally, Cíona.; Kelly, D.J., 2010: Tensile and compressive properties of fresh human carotid atherosclerotic plaques. Accurate characterisation of the mechanical properties of human atherosclerotic plaque is important for our understanding of the role of vascular mechanics in the development and treatment of atherosclerosis. The majority of previous studies inves...

Kalovidouris, A.; Mancuso, A.A.; Dillon, W., 1984: A CT-clinical approach to patients with symptoms related to the V, VII, IX-XII cranial nerves and cervical sympathetics. Forty-three patients who had signs and symptoms possibly related to the extracranial course of cranial nerves V, VII, IX, X-XII, and the cervical sympathetics were examined prospectively using high resolution CT to obtain images of thin sections d...

Bernat, G.A.; Johnson, Jr; Johnson, T.G., 1991: Distributional effects of household linkages. The distribution of household linkages determines how a change in the income of one household group affects the distribution of income among all households. The purpose of this paper is to demonstrate the large differences in household linkages in...

Ivany, Ja, 1975: Todays weed: Galinsoga. Weeds today: Winter 6 (1) 22

Sokolov, EA., 1979: Two new species of hide beetles (Coleoptera, Dermestidae) from Middle Asia. Anthrenus kryzhanovskii sp. nov. and A. zeravshanicus sp. nov. belong to the group of species with 4-jointed antennae. The larvae of A. kryzhanovskii occur in rock clefts where they feed on dead insects. The beetles feed in June on flowers of Umbe...

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