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Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells


, : Identification of calreticulin-like protein as one of the phosphoproteins modulated in response to oligogalacturonides in tobacco cells. Planta 02(3): 341-348

Oligogalacturonide-induced modifications of protein phosphorylation in cells of Nicotiana tabacum L. were investigated by in-vitro phosphorylation of plasma-membrane-enriched fractions and electrophoretic analysis on two-dimensional gels. About 100 polypeptides were resolved; among these 40 phosphoproteins were detected and their 33P-labelling quantified. Most of the phosphorylations were inhibited by staurosporine and several proteins were hyperphosphorylated in the presence of okadaic acid, indicating the presence of protein phosphatase(s) in addition to staurosporine-susceptible protein kinase(s) in the plasma-membrane-enriched fraction. In the presence of oligogalacturonides, phosphorylation of seven acidic polypeptides ranging from 15 to 65 kDa was strongly enhanced. A twofold enhancement of the phosphorylation of a 24-kDa protein and a two- to threefold decrease in the phosphorylation of acidic proteins of Mrs 62, 65, 80 and 84 was also observed in response to oligogalacturonides. One of the oligogalacturonide-modulated phosphoproteins was identified as calreticulin by direct nucleotide sequencing after preparative two-dimensional electrophoresis and comparison with protein database sequences. Decreased phosphorylation of calreticulin was also observed in vivo, shortly after addition of oligogalacturonides to tobacco cells, confirming the biological relevance of the modification. Although the presence of calreticulin, an abundent reticuloplasmin with high calcium-binding capacity, has been reported in both mammalian and plant cells its function is as yet largely unknown. Modulation of the phosphorylation of a plant calreticulin-like protein by oligogalacturonides is shown here, suggesting a role in the early transduction steps of this signal.

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Accession: 002863356

PMID: 9232905

DOI: 10.1007/s004250050136

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Mercado, R.; Toledo, M.; Pilar Leñero, M.; Trejos, M.; Miranda, A., 1993: A modified Strout test, used as a probe for the diagnosis of transplacental Chagas disease. I. Collection and preservation of the newborn's umbilical cord blood. Two umbilical cord (UC) blood samples are routinarily taken from each newborn (NB) at the University of Chile Maternity Hospital, to determine blood grouping and for detecting syphilis infection respectively. In august-september 1993, an additiona...

Huey J.M.; Kohman T.P., 1987: Mass spectrometric isotope dilution analysis of lead and thallium in the Allende Meteorite reference sample. Smithsonian Contributions to the Earth Sciences 27: 26

Henry, J. E., 1968: Malameba locustae and its antibiotic control in grasshopper cultures. The following is virtually the authors abstract. The trophozoites and cysts of the amoeba Malameba locustae, which infects grasshoppers in the laboratory in the United States and elsewhere, are described, and variations in the size of cysts measur...

Ramaswamy, K.; Radhakrishnan, A.N., 1966: Patterns of intestinal uptake & transport of amino acids in the rat. Indian Journal of Biochemistry 3(3): 138-143

Shil'nikova, V. K., 1969: On the release of nodule bacteria from leguminous plant nodules to the outside medium. Fragmentation of Rhizobium bacteroids into small bacterial cells was observed, using an electron microscope, in old nodules of red clover and lucerne during the last stages of functioning. These cells were then released into the nodule tissue; it...

Halberg E.; Pasley J.N.; Powell E.; Scheving L.E.; Halberg F., 1981: Suprachiasmatic nuclear circadian amplification and delay at different integration levels implications for breast cancer and psoriasis. International Journal of Chronobiology 7(4): 57

Smith, K.C.; Wang, T.C., 1989: recA-dependent DNA repair processes. UV-radiation-induced lesions in DNA result in the formation of: (1) excision gaps (i.e. a lesion is excised, leaving a gap), (2) daughter-strand gaps (i.e. a lesion can be skipped during replication, leaving a gap), and (3) double-strand breaks (i...

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Eisenberg B.R.; Kuda A.M.; Peter J.B., 1976: The effect of di aza cholesterol on the structure of rat skeletal muscle. Journal of Ultrastructure Research 55(2): 296

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