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Identification of carnation etched ring virus using polymerase chain reaction


, : Identification of carnation etched ring virus using polymerase chain reaction. Novenyvedelem 32(11): 553-556

For a rapid diagnosis of carnation etched ring caulimovirus (CERV) in carnation, one pair of primers was designed and synthesized from the conserved region of viral DNA. Using the primer, a 850-base pair long viral DNA was amplified, the PCR product was separated on agarose gel and found in different carnation samples. The method proved sensitive and allowed detection of CERV in 10 000-fold diluted plant sap.

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