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Identification of carnation etched ring virus using polymerase chain reaction


, : Identification of carnation etched ring virus using polymerase chain reaction. Novenyvedelem 32(11): 553-556

For a rapid diagnosis of carnation etched ring caulimovirus (CERV) in carnation, one pair of primers was designed and synthesized from the conserved region of viral DNA. Using the primer, a 850-base pair long viral DNA was amplified, the PCR product was separated on agarose gel and found in different carnation samples. The method proved sensitive and allowed detection of CERV in 10 000-fold diluted plant sap.

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Related references

Palkovics, L.; Balazs, E., 1996: Simple and rapid detection of carnation etched ring virus by polymerase chain reaction. Polymerase chain reaction was used to identify carnation etched ring virus (CERV) in leaves of infected carnations. Two 20-mer oligonucleotide primers were designed to the conserved intergenic sequence of carnation etched ring virus. The 850 nt am...

Hakkaart, F.A., 1977: The effect of carnation etched ring virus on the productivity of the carnation cultivar 'Joker'. Diseased plants flowered a week later than did healthy control plants, and flower quality was impaired.

Lawson, R.; Hearon, S.C.verolo, E., 1977: Carnation etched ring virus

Rana, G.L., 1984: Presence of carnation etched-ring virus in Apulia. Carnation plants of the Lonnolap variety, imported into Italy from France, which had chlorotic rings on the leaves, were subjected to microscopy, serological tests and transmission tests by means of Myzus persicae, which were allowed to feed on di...

Fujisawa, I.; Rubio-Huertos, M.; Matsui, C., 1971: Incorporation of thymidine-3H into Carnation etched ring virus. Evidence was obtained that CERV contains DNA and is a possible member of the cauliflower mosaic virus group. Leaves of Dianthus spp. infected with CERV contained characteristic X-bodies, smaller than nuclei and containing spherical particles 40-45...

Hull R.; Donson J., 1982: Physical mapping of the dna species of carnation etched ring virus and figwort mosaic virus. The native DNA of both carnation etched ring virus (CERV) and figwort mosaic virus (FMV) formed multiple bands on gel electrophoresis thus resembling cauliflower mosaic virus (CaMV) DNA; there were some minor differences in banding patterns. Denat...

Kuluev, B.R.; Chemeris, A.V., 2008: Amplification and cloning of dahlia mosaic virus and carnation etched ring virus promoters. Amplification and cloning of dahlia mosaic virus promoter were carried out for the first time. Sequence analysis showed homology between this promoter and the promoters of other caulimoviruses. In addition, amplification and cloning of the carnati...

Kuluev, B.R.; Chemeris, A.V., 2007: Amplification and cloning of dahlia mosaic virus and carnation etched ring virus promoters. Amplification and cloning of dahlia mosaic virus promoter were carried out for the first time. Sequence analysis showed homology between this promoter and the promoters of other caulimoviruses. In addition, amplification and cloning of the carnati...

Hull, R.; Sadler, J.; Longstaff, M., 1986: The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. Carnation etched ring virus (CERV) DNA comprises 7932 bp. CERV primer binding sites and overall genome organization are similar to those of the related cauliflower mosaic virus (CaMV). The six open reading frames of CERV showed amino acid homology...

Hakkaart, F.A.; Hartel, G., 1977: Research on etched ring virus in the carnation variety Joker. Plants infected with etched ring virus (which rarely causes obvious damage) and virus-free plants were obtained from the same clone of carnations, cv. Joker. Diseased plants started to flower 1 week later than healthy ones. They produced a slightl...