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Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS


, : Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS. Journal of Plant Growth Regulation 13(4): 183-186

In carrot crown gall cells transformed with Ti plasmids, Ti-derived IAA biosynthetic genes are transcribed and translated, followed by overproduction of IAA. However, the newly synthesized IAA is immediately metabolized to IAA-amino acid conjugate, and the content of endogenous free IAA is maintained at a low level. In this study, IAA-amino acid conjugate in carrot tissues transformed with Ti plasmids was identified as indole-3-acetylaspartic acid (IAAsp) by using frit-fast atom bombardment liquid chromatography/mass spectrometry (LC/MS).

Accession: 002863394

DOI: 10.1007/bf00226035

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Related references

Sasaki, K.S.kai, S.K.mada, H.H.rada, H., 1994: Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC. In carrot crown gall cells transformed with Ti plasmids, Ti-derived IAA biosynthetic genes are transcribed and translated, followed by overproduction of IAA. However, the newly synthesized IAA is immediately metabolized to IAA-amino acid conjugate...

Feung, C.S.; Hamilton, R.H.; Mumma, R.O., 1976: Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue. After 48 hours' incubation crown gall (Pseudomonas savastanoi) callus on Virginia creeper (Parthenocissus tricuspidata) had taken up 85-90% of the supplied IAA, and of that taken up 45% was conjugated with 5 amino acids. The significance of t...

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