+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS

Journal of Plant Growth Regulation 13(4): 183-186

Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS

In carrot crown gall cells transformed with Ti plasmids, Ti-derived IAA biosynthetic genes are transcribed and translated, followed by overproduction of IAA. However, the newly synthesized IAA is immediately metabolized to IAA-amino acid conjugate, and the content of endogenous free IAA is maintained at a low level. In this study, IAA-amino acid conjugate in carrot tissues transformed with Ti plasmids was identified as indole-3-acetylaspartic acid (IAAsp) by using frit-fast atom bombardment liquid chromatography/mass spectrometry (LC/MS).

Accession: 002863394

DOI: 10.1007/bf00226035

Download PDF Full Text: Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS

Related references

Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue. Plant Physiology 58(5): 666-669, 1976

Identification of 3-hydroxy-2-indolone-3-acetylaspartic acid as a new indole-3-acetic acid metabolite in Vicia roots. Plant and Cell Physiology 27(3): 559-562, 1986

Identification of 3-(O-beta-Glucosyl)-2-Indolone-3-Acetylaspartic Acid as a New Indole-3-Acetic Acid Metabolite in Vicia Seedlings. Plant Physiology 79(3): 667-671, 1985

Determination of indole-3-acetic acid and indole-3-acetylaspartic acid in pea plant with capillary electrophoresis and fluorescence detection. Journal of Chromatography, A 824(2): 231-239, 1998

Levels of endogenous indole 3 acetic acid and indole 3 acetylaspartic acid during adventitious root formation in pea cuttings. Physiologia Plantarum 82(4): 599-605, 1991

Indole-3-acetic acid and indole-3-acetylaspartic acid isolated from seeds of Heracleum laciniatum Horn. Plant growth regulation 10(2): 95-101, 1991

Biosynthesis and conjugation of indole-3-acetic acid in tobacco crown gall. Physiologia Plantarum 68(3): 458-466, 1986

On a case of sensitivity of Scorzonera crown-gall tissues to indole-acetic acid. C. R. Acad. Sci, Paris, 246: 13, 2023-2025, 1958

Primary Action of Indole-3-acetic Acid in Crown Gall Tumors: Increase of Solute Uptake. Plant Physiology 75(2): 354-358, 1984

Dynamics of indole-3-acetic acid oxidase activity in suspension culture of sunflower crown-gall. Acta Societatis Botanicorum Poloniae 53(1): 67-75, 1984