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Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS


Journal of Plant Growth Regulation 13(4): 183-186
Identification of conjugated IAA in carrot crown gall as indole-3-acetylaspartic acid (IAAsp) by LC/MS
In carrot crown gall cells transformed with Ti plasmids, Ti-derived IAA biosynthetic genes are transcribed and translated, followed by overproduction of IAA. However, the newly synthesized IAA is immediately metabolized to IAA-amino acid conjugate, and the content of endogenous free IAA is maintained at a low level. In this study, IAA-amino acid conjugate in carrot tissues transformed with Ti plasmids was identified as indole-3-acetylaspartic acid (IAAsp) by using frit-fast atom bombardment liquid chromatography/mass spectrometry (LC/MS).

Accession: 002863394

DOI: 10.1007/bf00226035

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