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Identification of dry-pinned museum specimens of the sibling species Anopheles gambiae and An. arabiensis (Diptera: Culicidae) using non-radioactively labelled DNA probes


, : Identification of dry-pinned museum specimens of the sibling species Anopheles gambiae and An. arabiensis (Diptera: Culicidae) using non-radioactively labelled DNA probes. Systematic Entomology 22(2): 173-180

Non-radioactively labelled DNA probes were tested for their ability to identify dried museum specimens of A. gambiae and its sibling species A. arabiensis. The specimens were the progeny of wild-caught females collected in 1991 from villages in western Kenya.

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Accession: 002863431

DOI: 10.1046/j.1365-3113.1997.d01-37.x

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Related references

Vij, R..; Harbach, R.E.; Service, MW.; Crampton, J.M.; Molyneux, DH., 1997: Identification of dry-pinned museum specimens of the sibling species Anopheles gambiae and An. arabiensis (Diptera: Culicidae) using the non-radioactively labelled DNA probes. Non-radioactively labelled DNA probes were tested for their ability to identify dried museum specimens of Anopheles gambiae and its sibling species An. arabiensis. The specimens were the progeny of wild-caught females collected in 1991 from villag...

Highton R.B.; Bryan J.H.; Boreham P.F.L.; Chandler J.A., 1979: Studies on the sibling species anopheles gambiae and anopheles arabiensis diptera culicidae in the kisumu area kenya. Seasonal abundance, feeding habits, resting habits and malaria infection rates of the sibling species A. gambiae and A. arabiensis were studied on the Kano Plain, Kenya and the surrounding foothills. A. arabiensis was the dominant species on the p...

Highton, RB.; Bryan, JH.; Boreham, PFL.; Chandler, JA., 1979: Studies on the sibling species Anopheles gambiae Giles and Anopheles arabiensis Patton (Diptera: Culicidae) in the Kisumu area, Kenya. A study was undertaken of the seasonal abundance, feeding habits, resting habits and malaria infection rates of the sibling species Anopheles gambiae Giles and A. arabiensis Patt. on the Kano Plain, Kenya, and the surrounding foothills. A. arabien...

Kirby, M.J.; Lindsay, S.W., 2004: Responses of adult mosquitoes of two sibling species, Anopheles arabiensis and A. gambiae s.s. (Diptera: Culicidae), to high temperatures. It is well known that amongst the sibling species of the Anopheles gambiae complex, A. arabiensis Patton predominates over A. gambiae sensu stricto Giles in hotter, drier parts of Africa. Here it was investigated whether A. arabiensis is better ad...

Copeland R.S.; Koros J.; Ouko M.; Taylor K.A.; Roberts C.R., 1992: Sensitivity of a ribosomal rna gene probe for identification of life stages of anopheles arabiensis and anopheles gambiae diptera culicidae using three storage methods. Individual larvae, pupae, female adults, and adult body parts of Anopheles arabiensis Patton and An. gambiae Giles were stored for 1 mo either in isopropanol at room temperature, over a desiccant at room temperature, or at -70.degree. C. DNA was e...

Townson, H..; Harbach, R.E.; Callan, T.A., 1999: DNA identification of museum specimens of the Anopheles gambiae complex: an evaluation of PCR as a tool for resolving the formal taxonomy of sibling species complexes. Species-specific rDNA primers were tested for their ability to identify museum specimens of the A. gambiae complex ranging in age from 15 to 93 years. 20 pinned females of known or inferred identity were selected for study from the collection of T...

Kampen, H.; Maier, W.A.; Seitz, H.M., 1996: Occurrence of an unusual polymerase chain reaction product during identification of Anopheles gambiae sibling species (Diptera: Culicidae). Using the polymerase chain reaction (PCR) for species differentiation within the Anopheles gambiae complex, we examined several hundred Cameroonian mosquito specimens. Applying an approved routine protocol for DNA extraction and PCR conditions, ap...

White G.B., 1985: Anopheles bwambae new species a malaria vector in the semliki valley uganda and its relationships with other sibling species of the anopheles gambiae complex diptera culicidae. The name Anopheles bwambae is proposed for the taxon previously called species D of the A. gambiae complex. This sibling species is known only from the vicinity of Buranga hot springs in Bwamba County, Toro District, Uganda, where it breeds in bra...

Copeland, R.S.; Koros, J.; Ouko, M.; Taylor, K.A.; Roberts, C.R., 1992: Sensitivity of a ribosomal RNA gene probe for identification of life stages of Anopheles arabiensis and An. gambiae (Diptera: Culicidae) using three storage methods. Individual larvae, pupae, female adults, and adult body parts of Anopheles arabiensis Patton and An. gambiae Giles were stored for 1 mo either in isopropanol at room temperature, over a desiccant at room temperature, or at -70 degrees C. DNA was e...

White, Gb, 1985: Anopheles bwambae sp.n., a malaria vector in the Semliki Valley, Uganda, and its relationships with other sibling species of the Anopheles gambiae complex (Diptera: Culicidae). Systematic entomology 10(4): 501-522