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Identification of enterotoxin-producing strains of Escherichia coli by PCR and biological methods


Veterinarni Medicina 42(4): 97-100
Identification of enterotoxin-producing strains of Escherichia coli by PCR and biological methods
Of 228 pig and calf E. coli isolates tested by the polymerase chain reaction (PCR), 129 were also examined for the production of the heat-labile enterotoxin (LT) in the Y1 cell line; the 2 tests yielded identical results in 125 (97%) strains. When the 228 strains were examined by the PCR and inoculation of newborn mice for the heat-stable enterotoxin (STa), this was detected by both methods in 53 strains.


Accession: 002863444

PMID: 9174393



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