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Identification of enterotoxin-producing strains of Escherichia coli by PCR and biological methods


, : Identification of enterotoxin-producing strains of Escherichia coli by PCR and biological methods. Veterinarni Medicina 42(4): 97-100

Of 228 pig and calf E. coli isolates tested by the polymerase chain reaction (PCR), 129 were also examined for the production of the heat-labile enterotoxin (LT) in the Y1 cell line; the 2 tests yielded identical results in 125 (97%) strains. When the 228 strains were examined by the PCR and inoculation of newborn mice for the heat-stable enterotoxin (STa), this was detected by both methods in 53 strains.

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Accession: 002863444

PMID: 9174393

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Franke, V., 1982: Identification of enterotoxin-producing strains of Escherichia coli from milk and milk products. Experiments showed that biochemical tests were insufficient to distinguish between toxin-producing and non-pathogenic strains of Escherichia coli. Serological tests were more effective than tissue cultures for diagnosis of heat labile (HL) toxin a...

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