+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of genes induced in alkaloid-producing cultures of Claviceps sp

, : Identification of genes induced in alkaloid-producing cultures of Claviceps sp. Current genetics1(4): 357-360

In order to identify genes which are expressed during alkaloid synthesis in an axenic culture of Claviceps sp. (strain ATCC 26245), a cDNA library from a producing culture was differentially screened with cDNA from producing (cDNA+) and non-producing (cDNA-) cultures, respectively. Altogether, ten cDNA clones were obtained, the alkaloid-synthesis-correlated expression of which was confirmed by Northern analyses. Evaluation of their nucleotide and derived amino-acid sequences identified one gene unequivocally, coding for dimethylallyltryptophan-synthase (DMAT-S), the initial enzyme of the specific alkaloid pathway. For two other genes significant homologies to known fungal genes were detected: one clone showed homology to the Neurospora crassa ccg1 gene, coding for a clock-regulated putative general stress protein: seven cDNA clones, derived from the same gene, which is highly expressed under these conditions, contained typical hydrophobin domains and long stretches of asparagine/glycine repeats (like QID3 from Trichoderma harzianum), thus probably representing a cell-wall constituent. These data show that this is not only a successful approach to clone genes specific for the alkaloid-pathway of C. purpurea, but also of genes which might be involved in the differentiation of sclerotial hyphae, the prerequisite for alkaloid synthesis.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863485

DOI: 10.1007/s002940050216

PDF Full Text: Identification of genes induced in alkaloid-producing cultures of Claviceps sp

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Alvarez-Silva, I.; Alvarez-Rodriguez, J.; Alvarez-Silva, S.; Perez-Echeverria, M.J.; Campayo-Martínez, A., 2007: Melancholic major depression and epilepsy. An analysis is carried out of a set of psychic phenomena appearing always in the same way: an experience suddenly invades the consciousness, unfolding automatically and with great intensity. This psychic automatism, of which the patient is a passi...

Tuttle, C.; McCune, J.; Benson, B., 1995: A system for automated microbial growth detection. American Clinical Laboratory 14(4): 6-7

Turrell, F.M.; Klotz, L.J., 1940: Density of stomata and oil glands and incidence of water spot in the rind of Washington Navel orange. From fresh free-hand sections of the proximal, equatorial, and distal areas of the rind of large and small Washington Navel orange fruits, the stomatal and oil gland density was detd. Stomatal density was greater in small fruits (15.66 per mm.2) t...

Pardasani D.; Fitt P.S., 1989: Study of the effect of mutations in dna repair genes on the thermal induction of error free repair in escherichia coli. Previous studies from this laboratory have shown that heat shock can induce a lon gene-dependent, error-free DNA repair process in certain strains of Escherichia coli. Further experiments have now shown that the phenomenon is dependent upon the re...

Natishvili O.G.; Tevzadze V.I., 2006: Expansion of a cohesive mud flow in debris cone. Meteorologiya i Gidrologiya 2006(7): 97-100

Ziegelbaum, M.; Thomas, A.; Zachary, A.A., 1987: The association of Peyronie's disease with HLA B7 cross-reactive antigens. A case report of identical twins. Cleveland Clinic Journal of Medicine 54(5): 427-430

Lafay, L.T.omas, F.M.nnen, L.C.arles, M.; Eschwege, E.B.rys, J.; Basdevant, A., 2001: Gender differences in the relation between food cravings and mood in an adult community: results from the Fleubaix Laventie Ville Sante study. International journal of eating disorders: 01 195-204

Teboul, V., 2015: Stimuli thresholds for isomerization-induced molecular motions in azobenzene-containing materials. We use large-scale molecular dynamics simulations of the isomerizations of azobenzene molecules diluted inside a simple molecular material to investigate the effect of a modification of the cis isomer shape on the induced diffusion mechanism. To t...

Ramiarina, R.; Almeida, R.Mvr.; Pereira, W.Ca., 2007: Hospital costs estimation and prediction as a function of patient and admission characteristics. The present work analyzed the association between hospital costs and patient admission characteristics in a general public hospital in the city of Rio de Janeiro, Brazil. The unit costs method was used to estimate inpatient day costs associated to...

Eberly, L.E.; Prineas, R.; Cohen, J.D.; Vazquez, G.; Zhi, X.; Neaton, J.D.; Kuller, L.H., 2005: Metabolic syndrome: risk factor distribution and 18-year mortality in the multiple risk factor intervention trial. To examine the long-term association of metabolic syndrome with mortality among those at high risk for cardiovascular disease (CVD). A total of 10,950 Multiple Risk Factor Intervention Trial (MRFIT) survivors were followed for mortality an additio...