EurekaMag.com logo
+ Translate

Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363


, : Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363. Applied and Environmental Microbiology 60(7): 2324-2329

The DNA sequence of the int-attP region of the small-isometric-headed lactococcal bacteriophage Tuc2009 is presented. In this region, an open reading frame, int, which potentially encodes a protein of 374 amino acids, representing the Tuc2009 integrase, was identified. The nucleotide sequence of the bacteriophage attachment site, attP, and the sequences of attB, attL, and attR in the lysogenic host Lactococcus lactis subsp. cremoris UC509 were determined. A sequence almost identical to the UC509 attB sequence was found to be present in the plasmid-free Tuc2009-resistant L. lactis subsp. cremoris MG1363. This site could be used for the site-specific integration of a plasmid carrying the Tuc2009 int-attP region in the chromosome of MG1363, thereby demonstrating that the application of chromosomal insertion vectors based on bacteriophage integration functions is not limited to the prophage-cured original host strain of the phage.

(PDF 0-2 workdays service)

Accession: 002863534

PMID: 8074513

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

van de Guchte, M.; Daly, C.; Fitzgerald, G.F.; Arendt, E.K., 1994: Identification of the putative repressor-encoding gene cI of the temperate lactococcal bacteriophage Tuc2009. The putative repressor-encoding gene cI of the temperate lactococcal bacteriophage Tuc2009 was cloned and sequenced. In the inferred amino-acid sequence, two domains can be recognized, one of which shows homology to DNA-binding domains of various...

Arendt, E.K.; Daly, C.; Fitzgerald, G.F.; van de Guchte, M., 1994: Molecular characterization of lactococcal bacteriophage Tuc2009 and identification and analysis of genes encoding lysin, a putative holin, and two structural proteins. Bacteriophage Tuc2009 is a temperate bacteriophage with a small isometric head and is isolated from Lactococcus lactis subsp. cremoris UC509. The phage genome is packaged by a headful mechanism, giving rise to circularly permuted molecules with te...

Kenny, J.G.; Leach, S.; de la Hoz, A.B.; Venema, G.; Kok, J.; Fitzgerald, G.F.; Nauta, A.; Alonso, J.C.; van Sinderen, D., 2006: Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009. Tuc2009 is a temperate bacteriophage of Lactococcus lactis subsp. cremoris UC509 which encodes a CI- and Cro-type lysogenic-lytic switch region. A helix-swap of the alpha 3 helices of the closely related CI-type proteins from the lactococcal phage...

Seegers, J.F.M.L.; M.G.ath, S.; O'Connell-Motherway, M.; Arendt, E.K.; van de Guchte, M.; Creaven, M.; Fitzgerald, G.F.; van Sinderen, D., 2004: Molecular and transcriptional analysis of the temperate lactococcal bacteriophage Tuc2009. The genome of bacteriophage Tuc2009 consists of 38 347 base pairs on which 57 open reading frames (ORFs) were identified, divided in two oppositely transcribed regions. The leftward-transcribed region harbors three ORFs, two of which are involved...

Sheehan, M.M.; García, J.L.; López, R.; García, P., 1996: Analysis of the catalytic domain of the lysin of the lactococcal bacteriophage Tuc2009 by chimeric gene assembling. An active chimeric cell wall lytic enzyme (Tsl) has been constructed by fusing the region coding for the N-terminal half of the lactococcal phage Tuc2009 lysin and the region coding for the C-terminal domain of the major pneumococcal autolysin. Th...

Sheehan, M.M.; Garcia, J.L.; Lopez, R.; Garcia, P., 1996: Analysis of the catalytic domain of the lysin of the lactococcal bacteriophage Tuc2009 by chimeric gene assembling. An active chimeric cell wall lytic enzyme (Tsl) has been constructed by fusing the region coding for the N-terminal half of the lactococcal phage Tuc2009 lysin and the region coding for the C-terminal domain of the major pneumococcal autolysin. Th...

L.B.urgeois, P.; Lautier, M.; van den Berghe, L.; Gasson, M.J.; Ritzenthaler, P., 1995: Physical and genetic map of the Lactococcus lactis subsp. cremoris MG1363 chromosome: comparison with that of Lactococcus lactis subsp. lactis IL 1403 reveals a large genome inversion. A physical and genetic map of the chromosome of the Lactococcus lactis subsp. cremoris reference strain MG1363 was established. The physical map was constructed for NotI, ApaI, and SmaI enzymes by using a strategy that combines creation of new rar...

Ainsworth, S.; Zomer, A.; Mahony, J.; van Sinderen, D., 2013: Lytic infection of Lactococcus lactis by bacteriophages Tuc2009 and c2 triggers alternative transcriptional host responses. Here we present an entire temporal transcriptional profile of Lactococcus lactis subsp. cremoris UC509.9 undergoing lytic infection with two distinct bacteriophages, Tuc2009 and c2. Furthermore, corresponding high-resolution whole-phage genome til...

Vegge, C.S.; Vogensen, F.K.; M.G.ath, S.; Neve, H.; van Sinderen, D.; Brøndsted, L., 2005: Identification of the lower baseplate protein as the antireceptor of the temperate lactococcal bacteriophages TP901-1 and Tuc2009. The first step in the infection process of tailed phages is recognition and binding to the host receptor. This interaction is mediated by the phage antireceptor located in the distal tail structure. The temperate Lactococcus lactis phage TP901-1 b...

Petzel J.P.; Mckay L.L., 1992: Molecular characterization of the integration of the lactose plasmid from lactococcus lactis ssp cremoris sk11 into the chromosome of lactococcus lactis ssp lactis. When Lactococcus lactis subsp. lactis LM0230 is transformed by the lactose plasmid (pSK11L) from Lactococcus lactis subsp. cremoris SK11, variants with pSK11L in the integrated state can be derived (J. M. Feirtag, J. P. Petzel, E. Pasalodos, K. A....