EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363


, : Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363. Applied and Environmental Microbiology 60(7): 2324-2329

The DNA sequence of the int-attP region of the small-isometric-headed lactococcal bacteriophage Tuc2009 is presented. In this region, an open reading frame, int, which potentially encodes a protein of 374 amino acids, representing the Tuc2009 integrase, was identified. The nucleotide sequence of the bacteriophage attachment site, attP, and the sequences of attB, attL, and attR in the lysogenic host Lactococcus lactis subsp. cremoris UC509 were determined. A sequence almost identical to the UC509 attB sequence was found to be present in the plasmid-free Tuc2009-resistant L. lactis subsp. cremoris MG1363. This site could be used for the site-specific integration of a plasmid carrying the Tuc2009 int-attP region in the chromosome of MG1363, thereby demonstrating that the application of chromosomal insertion vectors based on bacteriophage integration functions is not limited to the prophage-cured original host strain of the phage.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863534

PMID: 8074513

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Noble E.P.; Blum K.; Ritchie T.; Montgomery A.; Sheridan P.J., 1991: Allelic association of the d 2 dopamine receptor gene with receptor binding characteristics in alcoholism. The allelic association of the human D2 dopamine receptor gene with the binding characteristics of the D2 dopamine receptor was determined in 66 brains of alcoholic and nonalcoholic subjects. In a blinded experiment, DNA from the cerebral cortex w...

Van-Der-Linde, E. J.; Rong, I. H., 1997: New and interesting records of South African fungi. XV. Two new Laboulbeniales records from South Africa. The Laboulbeniales are predominantly parasites of true insects (Hexapoda), infesting mostly Coleoptera. Two members of the Laboulbeniales are reported from South Africa for the first time; Polyandromyces coptosomalis Thaxter and Laboulbenia monole...

Spitz T., 1991: Observations of short toed eagles circaetus gallicus new record and golden eagles aquila chrysaetos new record in the southern frankfurt oder district. Beitraege zur Vogelkunde 37(5-6): 352-353

Talesnick Mark; Shehadeh Sharbel, 2007: The effect of water content on the mechanical response of a high-porosity chalk. The paper deals with the effect of water content on the stress-strain response and compressive strength of a high-porosity chalk. Specimens were tested in three different testing configurations: uniaxial compression of solid cylinders, radial comp...

Manuel Christopher D.; Uren Norm F., 2000: Pre-stack depth migration of seismic multiples. Seismic multiples still present a major impediment to exploration on the North West Shelf of Western Australia. Considerable effort is being expended on the removal of multiple events from the seismic record to improve the quality of the image of...

Miller R.C.; Godfraind T., 1983: The action of tabernanthine on noradrenaline stimulated contractions and calcium 45 labeled movements in rat isolated vascular smooth muscle. The iboga alkaloid tabernanthine inhibited depolarization (100 mM K+)-induced contractions in aorta and mesenteric arteries in a concentration-dependent manner with the respective IC50 [median inhibitory concentration] values being .apprx. 21 and...

Poumay, Y.; Jolivet, G.; Pittelkow, M.R.; Herphelin, F.; De Potter, I.Y.; Mitev, V.; Houdebine, L.M., 1999: Human epidermal keratinocytes upregulate expression of the prolactin receptor after the onset of terminal differentiation, but do not respond to prolactin. Growing and differentiating keratinocytes maintain the epidermal barrier. This is partly controlled by growth factors and hormones. Prolactin (PRL) is named after its hormonal role in mammals during lactation, but is found in all vertebrates where...

Wrone, E.M.; Hornberger, J.M.; Zehnder, J.L.; McCann, L.M.; Coplon, N.S.; Fortmann, S.P., 2004: Randomized trial of folic acid for prevention of cardiovascular events in end-stage renal disease. High serum total homocysteine (tHcy) is gaining scrutiny as a risk factor for cardiovascular disease in the general population. The relationship between tHcy and mortality and cardiovascular events in patients with end-stage renal disease (ESRD) i...

Patterson, D.L.; Yunginger, J.W., 1994: Organic dust toxic syndrome in a child. Clinical Pediatrics 33(12): 743-745

Metzelaar M.J.; Korteweg J.; Sixma J.J.; Nieuwenhuis H.K., 1991: Biochemical characterization of pecam 1 cd31 antigen on human platelets. The platelet plasma membrane expresses several membrane glycoproteins with a high molecular weight. In this study we have investigated the properties of CD31 antigen on platelets and endothelial cells using the monoclonal antibody (MoAb) RUUU-PL 7...