EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins


, : Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins. Plant Journal 11(5): 1127-1140

Partial cDNA sequencing to obtain expressed sequence tags (ESTs) has led to the identification of tags to about 8000 of the estimated 20 000 genes in Arabidopsis thaliana. This figure represents four to five times the number of complete coding sequences from this organism available in international databases. In contrast to mammals, many proteins are encoded by multigene families in A. thaliana. Using ribosomal protein gene families as an example, it is possible to construct relatively long sequences from overlapping ESTs which are of sufficiently high quality to be able to unambiguously identify tags to individual members of multigene families, even when the sequences are highly conserved. A total of 106 genes encoding 50 different cytoplasmic ribosomal protein types have been identified, most proteins being encoded by at least two and up to four genes. Coding sequences of members of individual gene families are almost always very highly conserved and derived amino acid sequences are almost, if not completely, identical in the vast majority of cases. Sequence divergence is observed in untranslated regions which allows the definition of gene-specific probes. The method can be used to construct high-quality tags to any protein.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863585

DOI: 10.1046/j.1365-313x.1997.11051127.x

Download PDF Full Text: Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Linden, M., 2005: Illness is easier to tolerate the healthier one is. Have you mastered the art of salutary medicine?. Mmw Fortschritte der Medizin 147(37): 33-33

Um, K.; Bailey, M.; Clarke, A.; Chao, R., 1992: Concentration and identification of volatile compounds from heated beef fat using supercritical CO2 extraction-gas liquid chromatography. Beef fat heated at 100 degrees C was fractionated with supercritical CO2 into six fractions at 207 bar/50 degrees C and 345 bar/50 degrees C. The volatile compounds gas liquid chromatography/mass spectrometry. The concentration of volatiles from f...

Ayetey J. K., 1989: Landslides in Ghana and their economic significance. International Geological Congress, Abstracts--Congres Geologique Internationale, Resumes 28(1): 64

Cleaver, K.M.; Schreiber, G.A. .; Coulter, J.K.Reviewer, 1995: Supplement to Reversing the spiral (book review). Experimental Agriculture 31: 3-14

Sbruzzi, G.; Silveira, S.Azeredo.; Silva, D.Vidaletti.; Coronel, C.Correa.; Plentz, R.Della.Méa., 2012: Transcutaneous electrical nerve stimulation after thoracic surgery: systematic review and meta-analysis of 11 randomized trials. Avaliar os efeitos da estimula o el trica nervosa transcut nea (TENS) sobre a dor e a fun o pulmonar no p s-operat rio de cirurgias tor cicas por meio de uma revis o sistem tica e metan lise de estudos randomizados. A busca incluiu as bases MEDLIN...

Iannicelli, E.; Chianta, G.A.; Salvini, V.; Almberger, M.; Monacelli, G.; Passariello, R., 2000: Evaluation of bifid median nerve with sonography and MR imaging. Bifid median nerve is an anatomic variation that may be associated with carpal tunnel syndrome. It is important for the surgeon to be aware of the existence of this condition preoperatively in order to plan the carpal tunnel release. We report the...

Chun-Xian WU.J.n-Jun WANG.S.-Zhi ZHANG.Z.ong-Ming ZHANG, 2011: Adsorption and Desorption of Methiopyrsulfuron in Soils. Methiopyrsulfuron is a new low-rate sulfonylurea herbicide for weed control in wheat; however, there is a lack of published information on its behavior in soils. In this study, methiopyrsulfuron adsorption and desorption were measured in seven soi...

Park, I.J.; Kim, H.C.; Yu, C.S.; Kim, J.S.; Jang, S.J.; Kim, J.C., 2006: Low-grade fibromyxoid sarcoma of the colon. Low-grade fibromyxoid sarcoma is a recently recognised soft tissue neoplasm. It is rare and has a tendency to arise from deep soft tissue of the lower extremities. An origin from the colon has not been reported in the medical literature. We repor...

Hammar, J.; Dempson, JB.; Verspoor, E., 1991: Natural hybridization between Arctic char (Salvelinus alpinus) and brook trout (S. fontinalis): evidence from northern Labrador. Electrophoretic analysis of successive samples of Salvelinus collected in the Fraser River watershed of northern Labrador in 1984 and 1986 revealed the existence of hybrids between Arctic char and brook trout. 11 electrophoretic loci were studied...

Götz, I.; Götz, M., 2006: How and why parents change their attitudes to prenatal diagnosis. Parents who know they are carriers of a genetically transmitted disease because they gave birth to an affected child are being offered a number of reproductive options in a subsequent pregnancy. We describe the attitudes and motives of parents of...