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Identification of mycoplasmas in cell culture by immunobinding with a conjugate of protein A and colloidal gold


, : Identification of mycoplasmas in cell culture by immunobinding with a conjugate of protein A and colloidal gold. Revista de Salud Animal 17(3): 291-294

Mycoplasma hyorhinis was distinguished from M. arginini and M. salivarium-M.hominis by using the colloidal gold conjugate in the immunobinding technique of H. Kotani & G.


Accession: 002863612

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Related references

Singh, V.P.; Srivastava, N.C.; Singh, V.P., 1999: Identification of mycoplasmas by immunobinding assay. The immunobinding assay (IBA) was standardized for rapid identification of various members of Mycoplasma mycoides spp. and other mycoplasmas including M. bovis, M. agalactiae, M. gallinarum and A. laidlawi. A 2 micro l sample of mycoplasma broth c...

Kotani, H.; Huang, H.; McGarrity, G.J., 1987: Identification and isolation of mycoplasmas by immunobinding. This laboratory has developed an immunobinding assay (IBA) to identify and detect mycoplasmas in a variety of specimens. The specimen is inoculated in volumes of 10 microliters onto nitrocellulose (NC) paper, which is then blocked, fixed, and incu...

Kotani H.; Mcgarrity G.J., 1985: Rapid and simple identification of mycoplasmas by immunobinding. A simple and rapid method of species identification of mycoplasmas by immunobinding assay is described. Small amounts of antigen of supernatant from cell cultures, broth cultures or clinical specimens were spotted onto nitrocellulose paper. This w...

Poumarat, Francois, 1998: Identification of mycoplasmas by dot immunobinding on membrane filtration. Miles, R [Editor], Nicholas, R [Editor] Methods in Molecular Biology; Mycoplasma protocols 113-118

Poumarat, F., 1998: Identification of mycoplasmas by dot immunobinding on membrane filtration (MF dot). Methods in Molecular Biology 104: 113-118

Poumarat, F.; Perrin, B.; Longchambon, D., 1991: Identification of ruminant mycoplasmas by dot immunobinding on membrane filtration (MF dot). A simple method of species identification of ruminant mycoplasmas by dot immunobinding (MF dot) is described. Mycoplasmas from broth cultures were directly trapped onto the surface of low protein-binding affinity membranes by vacuum filtration. Sp...

Chennuru, S.; Pavuluri, P.Rao., 2016: Flow-Through Assay for Detection of Antibodies Using Protein-A Colloidal Gold Conjugate as a Probe. Flow-through assay (FTA) is a rapid, simple-to-perform, cost-effective, and user-friendly diagnostic test for monitoring infections in non-laboratory settings. It is mostly applied for antibody detection. FTA employing protein-A colloidal gold con...

Monsigny, M.; Midoux, P.; Depierreux, C.; Bebear, C.; L.B.is, M.T.; Valeur, B., 1990: Benzoxazinone kanamycin A conjugate. A new fluorescent probe suitable to detect mycoplasmas in cell culture. The synthesis of a new benzoxazinone derivative suitable to detect early infection of cultured cells with mycoplasmas is described. p-[.beta.-(7-dimethylamino 1,4-benzoxazin 2-one 3yl) - vinyl]-phenylpropenoic acid was coupled to kanamycin A, an a...

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