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Identification of nematode-trapping fungi using RFLP analysis of the PCR-amplified ITS region of ribosomal DNA

, : Identification of nematode-trapping fungi using RFLP analysis of the PCR-amplified ITS region of ribosomal DNA. Mycological Research 100(5): 531-534

Six isolates of Arthrobotrys oligospora var. oligospora, identified by morphological characters, were screened for polymorphism in the ITS region of the ribosomal DNA. RFLPs produced with four different endonucleases were identical for five out of six isolates. The RFLP patterns were also compared to those from the ITS regions of other Arthrobotrys species as well as to other nematophagous fungi. All species tested produced unique RFLP patterns which could be distinguished from each other easily. The results suggest this method to be a good tool for fast identification of large numbers of isolates in screening assays. Only minute colonies are needed since enough amplification product could be obtained from 0.1 ng of total genomic DNA. The method can also be useful in verifying the true identity of isolates which are difficult to type morphologically.

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Accession: 002863617

DOI: 10.1016/s0953-7562(96)80004-9

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Soballe D.M.; Kimmel B.L., 1987: A large scale comparison of factors influencing phytoplankton abundance in rivers lakes and impoundments. We analyzed two large data sets (the National Stream Water Quality Accounting Network and the National Eutrophication Survey [USA]) to compare quantitatively the ecological structure and function of rivers, river impoundments, and natural lakes on...

Watson E.L.; Dowd F., 1981: Effect of sodium ions on cyclic amp and cyclic gmp levels in mouse parotid acini. Federation Proceedings 40(3 PART 1): 655

Buchenauer, H.; Erwin, D.C., 1971: Control of Verticillium wilt of Cotton by spraying foliage with benomyl and thiabendazole solubilized with hydrochloric acid. Will was prevented in plants sprayed twice with TBZ (pH 2.7) and benomyl (1-5) at 2500-10, 000 ppm before inoculation. No phytotoxicity was observed and both treatments were curative when applied to inoculated plants showing initial symptoms. TBZ...

Somers, C. J., 1985: The presence of infective larvae of Dictyocaulus viviparus inside sporangia of the fungus Pilobolus kleinii. Visual evidence for the occurrence of D. viviparus L3 inside sporangia of P. kleinii following ejection of the sporangia from the sporangiophores is presented. The results show no evidence for the transport of D. viviparus L3 on the outer surface...

Baker, L. C., 1942: The nation's food. 5. Meat as food. 5. The effect on meat of cooking and processing. Changes which occur in the composition of meat on cooking and their effect on the nutritive value of the meat are discussed.

Anonymous, 1981: Some social aspects of dentistry. Social Science & Medicine. Part A, Medical Sociology 15(6): 743-823

Aziz-Zadeh, L.; Sheng, T.; Gheytanchi, A., 2010: Common premotor regions for the perception and production of prosody and correlations with empathy and prosodic ability. Prosody, the melody and intonation of speech, involves the rhythm, rate, pitch and voice quality to relay linguistic and emotional information from one individual to another. A significant component of human social communication depends upon inter...

Ryabinin, A.E.; Wang, Y.M.; Bachtell, R.K.; Kinney, A.E.; Grubb, M.C.; Mark, G.P., 1999: Pentobarbital anesthesia blocks cocaine- and alcohol-induced immediate early gene expression. Society for Neuroscience Abstracts 25(1-2): 812

Ringe, B.; Braun, F.; Wietzke, P.; Oellerich, M.; Ramadori, G., 2000: Replacement of corticosteroids by mycophenolate mofetil in liver graft recipients on initial tacrolimus immunosuppression. Transplantation Proceedings 32(7): 2543-2543

Pasargiklian M.; Ghiringhelli G., 1969: Ethanol and the respiratory apparatus physio pathological and clinical considerations. Metabolismo 5(5): 429-440