EurekaMag.com logo
+ Translate

Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus, and expression of corresponding mRNAs in developing root nodules


, : Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus, and expression of corresponding mRNAs in developing root nodules. Plant journal: for cell and molecular biology 11(2): 237-250

In this study, 266 cDNA clones were isolated from a cDNA library made from mRNA of three-week-old root nodules of Lotus japonicus, employing a degenerate oligonucleotide probe that corresponds to a conserved region of small GTP-binding (SMG) proteins. The clones were sorted into groups by cross hybridization and 3' sequencing, and 33 contigs were sequenced in an orderly fashion. Twenty-seven complete and six incomplete protein structures were deduced, which represent three subfamilies of the superfamily of signal transducing GTP-binding proteins. The 33 proteins are divided into nine subclasses, of which seven belong to the Ypt/Rab subfamily, one subclass represents the Rho/Rac subfamily, and one subclass represents the Ran subfamily of small GTP-binding proteins. The protein sequences were compared with related proteins from other plants, from mammals and other species, and discussed with respect to structure and function in different cellular processes. It is apparent that the number of genes encoding SMG proteins in plants must be quite large, since the large number of subclasses found in other eukaryotes is not fully represented in our analysis. Transcription patterns through root nodule development were analysed for 27 of the 33 cDNAs. Differential expression patterns may reflect whether the coded gene product is of importance for organ development. Most mRNAs appear to be constitutively expressed; however, a few unique mRNAs representing the subclasses Rab1, Rab2, Rab5, Rab7 and Rac show elevated levels in root nodules, and certain Rab7, Rab8 and Rab11 species are enriched in aerial parts of the plant. This suggests that most small GTPases have household functions, whereas a few may be required for specialized activities that are important for specialized cells.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863627

PMID: 9076991

DOI: 10.1046/j.1365-313x.1997.11020237.x

Download PDF Full Text: Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus, and expression of corresponding mRNAs in developing root nodules


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Olivieri R.; Serpagli E., 1990: Latest silurian early devonian conodonts from the mason porcus section near fluminimaggiore southwestern sardinia italy. Several conodant species, some reported for the first time in Europe and in Sardinia, are described and illustrated from the Uppermost Silurian-Lower Devonian beds of Southwestern Sardinia. Two species (Pedavis palmatus and Sannemannia ishnusae),...

Bui, H.; Haukoos, J.; Donayre, C.; White, R.; de Virgilio, C., 2004: Predictors of cardiac morbidity and mortality in patients undergoing endovascular repair of the thoracic aorta. The aim of this study was to determine predictors of cardiac morbidity and mortality in patients undergoing endovascular repair of the thoracic aorta. This was a retrospective cohort study that took place in a University-affiliated county hospital...

Fazakas, J.; Marinescu, D.M., 1983: Determination of thallium in cadmium and lead by graphite-furnace atomic-absorption spectrometry with sample vaporization from a platform. When the sample is vaporized from the wall of a graphite furnace it is not possible to determine thallium in cadmium and lead by AAS without matrix matching of the standards. In the case of a lead matrix and vaporization from the wall, the thalliu...

Paricio N.; Pascual L.; Martinez Sebastian M.J.; Perx Alonso M.; D.F.utos R., 1990: Homologous sequences to the p transposable element in drosophila subobscura. The distribution of P homologous sequences among different strains of D. subobscura is described. Southern analysis of genomic DNAs from the strains restricted with different enzymes showed rather homogeneous hybridization patterns. P homologous s...

Ferreri, M.; Rouillon, F.; Nuss, P.; Bazin, N.; Farah, S.; Djaballah, K.; Gerard, D., 2001: What information do patients with schizophrenia have about their illness and treatment?. Improved compliance with antipsychotic medication is a major issue in schizophrenic management. For this purpose educational programs have been used, but up to now, little or no information has been gathered or published in France concerning schiz...

Emami, E.; Wootton, J.; Galarneau, C.; Bedos, C., 2014: Oral health and access to dental care: a qualitative exploration in rural Quebec. We sought to explore how rural residents perceive their oral health and their access to dental care. We conducted a qualitative research study in rural Quebec. We used purposeful sampling to recruit study participants. A trained interviewer conduc...

Emenhiser, C.; Lovalvo, J.L.; Rock, C.L.; Ruffin, M.T.; Schwartz, S.J., 1996: Geometrical beta-carotene isomers in human cervical tissue. FASEB Journal 10(3): A242

Shu, S.; Liu, X.; Korn, E.D., 2002: The assembly domain of Dictyostelium myosin II localizes to the cleavage furrow of dividing cells. Molecular Biology of the Cell 13(Supplement): 454a, Nov

Magalhães, M.M.; Alvim, E.de.F., 1967: Occupational health: a wide open field in the nursing profession. Revista Brasileira de Enfermagem 20(4): 373-385

Windle, J., 2005: To triage, or not to triage.... Emergency Nurse 13(2): 11-11