EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of novel species-specific antigens of Mycoplasma hyopneumoniae by preparative SDS-PAGE ELISA profiling


Microbiology 143: 663-673
Identification of novel species-specific antigens of Mycoplasma hyopneumoniae by preparative SDS-PAGE ELISA profiling
Mycoplasma hyopneumoniae, M. hyorhinis and M. flocculare are commonly isolated from the respiratory tract of pigs and are phylogenetically related. The identification and characterization of antigens specific for M. hyopneumoniae is crucial for the development of serological reagents and for understanding the mechanisms of pathogenicity of this pathogen. Protein and antigen profiles of six strains of M. hyopneumoniae, four strains of M. hyorhinis and a type strain of M. flocculare were compared using SDS-PAGE and immunoblotting. Five strains of M. hyopneumoniae originally isolated from diverse geographical regions produced similar protein and antigen profiles. One strain, C1735/2, produced a unique protein profile and was poorly immunoreactive, suggesting that some strains of M. hyopneumoniae may possess a structurally modified repertoire of antigens. Major M. hyopneumoniae antigens with molecular masses of approximately 36, 43, 48, 52, 76, 78, 80, 82, 94, 106, 114 and 200 kDa were identified by immunoblotting using hyperimmune pig sera raised against both high and low passage strains of M. hyopneumoniae. Porcine hyperimmune sera raised against the GDL type strain of M. hyorhinis reacted strongly with all M. hyorhinis strains although the profiles displayed considerable variation. Major antigens of molecular mass 42, 49, 52, 78, 80 and 82 kDa were identified in type strains GDL and BTS-7 and field strain 2; however, field strain 1 produced a unique profile. A preparative SDS-PAGE profiling (PPP) technique was developed which enabled quantification of the immunoreactivity of denatured antigens with porcine serum by ELISA. PPP facilitated the rapid identification of species-specific and cross-reactive antigens among the three mycoplasma species. PPP studies revealed several strongly immunoreactive M. hyopneumoniae-specific antigens of 43, 76, 94, 114 and 200 kDa as well as antigens of molecular mass between 52 and 62 kDa which were not apparent in immunoblotting studies. Rabbit monospecific anti-42 kDa serum reacted specifically with a 43 kDa antigen in whole cell lysates of geographically diverse strains of M. hyopneumoniae and failed to cross-react with M. flocculare or M. hyorhinis whole cell lysates. This study has identified a number of M. hyopneumoniae-specific antigens which warrant further investigation to determine their potential as diagnostic reagents and the role they play, if any, in pathogenicity.


Accession: 002863638

PMID: 9043142

DOI: 10.1099/00221287-143-2-663



Related references

Preparative electroelution of specific protein antigens from Mycoplasma pneumoniae: use in an enzyme-linked immunosorbent assay (ELISA). Annales de L'institut Pasteur. Microbiology 139(5): 589-603, 1988

Species specific antigens of mycoplasma hyopneumoniae and cross reactions with other porcine mycoplasmas. Current Microbiology 15(4): 233-239, 1987

Detection of antibodies against Mycoplasma hyopneumoniae in pig farms by a competitive ELISA (CIVTEST SUIS MYCOPLASMA HYOPNEUMONIAE). Revista de Salud Animal 27(1): 21-25, 2005

Determination of antigenic characteristics of mycoplasma hyopneumoniae by elisa ii. the stabilities of mycoplasma hyopneumoniae antigen. Journal of the Chinese Society of Veterinary Science 15(3): 199-206, 1989

Restriction endo nuclease analyses of 2 porcine mycoplasma dna species sequence specific methylation in the mycoplasma hyopneumoniae genome. International Journal of Systematic Bacteriology 34(1): 16-20, 1984

Serological cross reactivity of porcine reference anti sera to mycoplasma hyopneumoniae mycoplasma flocculare mycoplasma hyorhinis and mycoplasma hyosynoviae indicated by the enzyme linked immuno sorbent assay elisa complement fixation and indirect hem agglutination tests. Canadian Journal of Comparative Medicine 48(2): 202-207, 1984

Immune responses of a chimaeric protein vaccine containing Mycoplasma hyopneumoniae antigens and LTB against experimental M. hyopneumoniae infection in pigs. Vaccine 32(36): 4689-4694, 2015

Evaluation of serovar-independent ELISA antigens of Actinobacillus pleuropneumoniae in pigs, following experimental challenge with A. pleuropneumoniae, Mycoplasma hyopneumoniae and Pasteurella multocida. Australian Veterinary Journal 90(6): 225-234, 2012

Determination of antigenic characteristics of mycoplasma hyopneumoniae by elisa i. the establishment and performance of elisa. Journal of the Chinese Society of Veterinary Science 15(3): 193-198, 1989

Use of antibodies against the P36 protein of Mycoplasma hyopneumoniae for the identification of M. hyopneumoniae strains. Molecular and Cellular Probes 5(6): 451-457, 1991