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Identification of ovule-enriched/specific proteins in Cycas taiwaniana using two-dimensional electrophoresis and blotting


, : Identification of ovule-enriched/specific proteins in Cycas taiwaniana using two-dimensional electrophoresis and blotting. Botanical Bulletin of Academia Sinica (Taipei) 38(2): 109-114

We utilized two-dimensional polyacrylamide gel electrophoresis, coupled with silver stain, to identify five ovule-enriched and three ovule-specific polypeptides in Cycas taiwaniana Carr. (CTO). These ovule-enriched and -specific proteins are all with acidic pIs in the 4.8-6.4 range. Polyclonal antiserum to an ovule polypeptide (CTO-22) was prepared and affinity-purified to investigate the specificity of the protein. Immunoblot analyses of total protein from the vegetative organs and from organs of the strobili demonstrated that the CTO-22 polypeptide was ovule-specific. The amino terminal sequence of the CTO-22 protein exhibits no similarity with any known protein. To our knowledge, this is the first ovule-specific protein reported in gymnosperms.

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