EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of peptides released from casein micelles by limited trypsinolysis


, : Identification of peptides released from casein micelles by limited trypsinolysis. Journal of Agricultural & Food Chemistry 44(9): 2517-2522

Bovine casein micelles were treated with low concentrations of trypsin before and after heating. Peptides present in the supernatant solutions of time course samples after high-speed centrifugation were separated by reversed-phase HPLC and identified principally by mass spectrometry. beta-Casein was hydrolyzed rapidly, the initial cleavages occurring in the region of the plasmin-sensitive bonds. alpha-s1-Casein was hydrolyzed more slowly, the initial cleavage points being in the N-terminal region, but some trypsin-sensitive bonds in this part of the molecule appeared to be shielded. alpha-S2-Casein was hydrolyzed slowly from the C-terminal. Hydrolysis of kappa-casein was insignificant. No peptides containing phosphoserine clusters were released. Although heating increased the rate of hydrolysis of all of the caseins, the HPLC profiles were generally similar to those obtained with unheated micelles. The results are discussed in relation to the possible structure of the surface of the casein micelle.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863677

DOI: 10.1021/jf950832u

PDF Full Text: Identification of peptides released from casein micelles by limited trypsinolysis


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Zogaris, S.; Economou, A.N.; Dimopoulos, P., 2009: Ecoregions in the southern balkans: should their boundaries be revised?. Ecoregion delineations have gained increased attention in Europe, especially following the Water Framework Directive 2000/60/EC (WFD), which provides the European Union's first policy-relevant ecoregion map. However, the WFD's ecoregions...

Kumar, N.; Arumugam, R.; Kandasamy, O.S., 1982: The effect of NPK on flower production of pyrethrum (Chrysanthemum cinerariifolium Vis.). In 2-year trials on soil with medium to high available N and low P and K, N, P2O5 and K2O were applied at 20:40-120:20 kg/ha. Plant height, plant spread and flower yield were greatest at 20:120:20 kg/ha.

Baker T., 1994: The geology of the Eloise copper-gold deposit, NW Queensland, Australia. Publication Series - Australasian Institute of Mining and Metallurgy 5/94: 199-205

Smutna, M.; Drastichova, J.; Svobodova, Z.; Vorlova, L., 2003: Immunoglobulins of fishes - comparative biochemistry (a review). Various features and aspects of the immune system of fishes are presented and discussed, including both the nonspecific and specific immune system, as well as both the cellular and humoral components. The article also elaborates on the five basic...

Essenberg R.C., 1990: A map of the genome of brucella abortus strain 19. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 197

Dykstra M.A.; Walker C.A., 1979: Comparison of enterotube ii with enterotube and api 20e for identification of enterobacteriaceae. Abstracts of the Annual Meeting of the American Society for Microbiology (79): 334

Bains, S.N.; Judson, M.A., 2013: Allergic bronchopulmonary aspergillosis. Allergic bronchopulmonary aspergillosis (ABPA) is caused by an exaggerated T(H)2 response to the ubiquitous mold Aspergillus fumigatus. ABPA develops in a small fraction of patients with cystic fibrosis and asthma, suggesting that intrinsic host d...

Belfi, C.A.; Chatterjee, S.; Berger, N.A., 1998: Induction of GRP78 stress protein is associated with a decrease in Topo IIalpha in human tumor xenografts. Proceedings of the American Association for Cancer Research Annual Meeting 39: 424, March

Matzopoulos, R.G.; Truen, S.; Bowman, B.; Corrigall, J., 2014: The cost of harmful alcohol use in South Africa. The economic, social and health costs associated with alcohol-related harms are important measures with which to inform alcohol management policies and laws. This analysis builds on previous cost estimates for South Africa. We reviewed existing in...

Hu, F-Wei.; Hosomichi, J.; Kanno, Z.; Soma, K., 2009: The influence of occlusal stimuli on basic fibroblast growth factor expression in the periodontal healing of replanted teeth. Occlusal stimuli and the periodontal healing of replanted teeth have been reported to be related. However, the mechanism for preventing dentoalveolar ankylosis remains unclear. Basic fibroblast growth factor (bFGF/FGF-2) is considered as a key fac...