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Identification of peptides released from casein micelles by limited trypsinolysis


, : Identification of peptides released from casein micelles by limited trypsinolysis. Journal of Agricultural & Food Chemistry 44(9): 2517-2522

Bovine casein micelles were treated with low concentrations of trypsin before and after heating. Peptides present in the supernatant solutions of time course samples after high-speed centrifugation were separated by reversed-phase HPLC and identified principally by mass spectrometry. beta-Casein was hydrolyzed rapidly, the initial cleavages occurring in the region of the plasmin-sensitive bonds. alpha-s1-Casein was hydrolyzed more slowly, the initial cleavage points being in the N-terminal region, but some trypsin-sensitive bonds in this part of the molecule appeared to be shielded. alpha-S2-Casein was hydrolyzed slowly from the C-terminal. Hydrolysis of kappa-casein was insignificant. No peptides containing phosphoserine clusters were released. Although heating increased the rate of hydrolysis of all of the caseins, the HPLC profiles were generally similar to those obtained with unheated micelles. The results are discussed in relation to the possible structure of the surface of the casein micelle.

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Accession: 002863677

DOI: 10.1021/jf950832u

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Maia V.; Pathak S.; Blasdel T.L.; Flanagan J.P., 1987: Karyotypic analysis of the scimitar horned oryx oryx tao bovidae artiodactyla. The diploid chromosome number in the scimitar-horn oryx is 2n = 58. The karyotype consists of acrocentric chromosomes except for a pair of autosomes which is subtelocentric. The X is a large acrocentric and the Y is the smallest-sized acrocentric...

Sippel, T.O., 1971: Histochemistry of protein sulfhydryl and disulfide groups in the lens. Experimental Eye Research 11(3): 383-388

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Chen, C.C.; Fan, P.C., 1977: The effect of diethylcarbamazine treatment on the development of bancroftian microfilariae in Culex p. fatigans. Bancroftian microfilariae [Wuchereria bancrofti] survived after one to three courses of diethylcarbamazine treatment in human carriers, and the larvae were able to reach the infective stage of Culex pipiens fatigans Wied that fed on them. The infe...

Voumvourakis, C.; Rombos, A.; Konstantoulakis, M.M.; Segditsa, I.; Papageorgiou, C., 1992: Serum anti-GM-1 and anti-GD-1a antibodies in patients with motor neuron disease. Using an enzyme-linked immunosorbent assay (ELISA) sera from 100 individuals, 20 with motor neuron (MND), 25 with peripheral neuropathy (NP), 15 with degenerative dementia and 40 controls, were examined in order to detect serum IgM and IgG anti-GM...

Guan, E.; Burgess, W.H.; Robinson, S.L., 1991: Phagocytic cell molecules that bind the collagen-like region of Clq. Involvement in the Clq-mediated enhancement of phagocytosis. The Journal of Biological Chemistry 266: 345-55

Ciurus, J.D.ozdz, A.K.upinski, J.J.ckowska, H., 1983: Growth and slaughter performance of commercial crossing of Polish mountain ewes with rams of meat-type and Friesian breeds. Zeszyty problemowe postepow nauk rolniczych: 65) 77-86

Ren, Z-Gang.; Yang, J-Yi.; Song, Y-Lin.; Li, N-Ya.; Li, H-Xi.; Chen, Y.; Zhang, Y.; Lang, J-Ping., 2009: From trans-[(eta5-C5Me5)2Mo2S2(mu-S)2] to [(eta5-C5Me5)2Mo2(mu3-S)4(CuMeCN)2]2+ to [(eta5-C5Me5)2Mo2(mu3-S)4Cu2]-based polymeric and dimeric clusters: syntheses, structures and enhanced third-order non-linear optical performances. Reactions of trans-[(eta5-C5Me5)2Mo2S2(mu-S)2] (1) with 2 equiv. of [Cu(MeCN)4]X (X = PF6-, ClO4-) produced two acetonitrile-coordinated cubane-like cationic clusters [(eta5-C5Me5)2Mo2(mu3-S)4Cu2(MeCN)2]X2 (X = PF6- (2), ClO4- (3)). Treatment of 2...

Lausten, G.S.; Anagnostaki, L.; Thomsen, O.F., 1984: Inverted papilloma of the upper urinary tract. Four cases of inverted papilloma in the upper urinary tract are reported-three were located in the ureter and one in the renal pelvis. Clinical and pathological findings are described. In two cases the patients had a history of transitional cell c...

Nicolet J., 1968: On pig hemophilosis i identification of a frequent agent haemophilus parahaemolyticus haemophilus pleuropneumoniae haemophilus suis. Pathologia et Microbiologia 31(4): 215-225