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Identification of phytoplasmas in Alstroemeria

, : Identification of phytoplasmas in Alstroemeria. Acta Horticulturae ( 432): 312-317

During a survey of viruses infecting Alstroemeria, plants showing virescent and malformed flowers were found under greenhouse conditions. After DNA extraction, 17 samples were tested with direct and nested-PCR using several phytoplasma 16S ribosomal primer pairs. In 5 samples the presence of a 1.2 kb DNA product identical to those observed in phytoplasma strain control DNAs was detected. No amplification was observed in the other Alstroemeria samples or the negative controls.

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