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Identification of regions in HIV-1 Nef required for efficient downregulation of cell surface CD4

, : Identification of regions in HIV-1 Nef required for efficient downregulation of cell surface CD4. Virology 231(2): 231-238

Downregulation of call surface CD4 is a characteristic property of all lentiviral Nef proteins. We have used mutational analysis to define regions within HIV-1 Nef that are critical for this biological activity. Two discontinuous regions In Nef, extending approximately from residues 96 to 144 and from residues 175 to 186, are reported to be essential for efficient CD4 downregulation. Interestingly, these sequences coincide with two conserved regions of the Nef protein that are juxtaposed to form a single surface on the known structure of Nef. A third, more amino terminal conserved region in Nef, previously reported to be important for Nef enhancement of virion infectivity, was found to be largely dispensable for CD4 downregulation. These data raise the possibility that Nef may contain two structurally distinct functional domains, only one of which contributes to the CD4 downregulation phenotype.

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Accession: 002863738

PMID: 9168885

DOI: 10.1006/viro.1997.8517

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Other references

Tack M.; Cherniack N.S.; Altose M.D., 1982: Effect of age on the accuracy of inspired volume reproduction. Federation Proceedings 41(4): ABSTRACT 6494

Ghosh, A.K.; Schuster, G.B., 2006: Role of the guanine N1 imino proton in the migration and reaction of radical cations in DNA oligomers. Oxidation of a guanine nucleobase to its radical cation in DNA oligomers causes an increase in the acidity of the N1 imino proton that may lead to its spontaneous transfer to N3 of the paired cytosine. This proton transfer is suspected of playing...

Drumheller, P.D.; Hubbell, J.A., 1991: Local modulation of intracellular calcium levels near a single-cell wound in human endothelial monolayers. An endothelial cell monolayer with a single mechanically lysed cell was used as a model to examine the extent, kinetics, and nature of local calcium mobilization in the neighborhood of a wound. Individual endothelial cells from confluent monolayer...

Culp W.; Morrisey J.; Obrig T.; M.K.ehan W.; Hardesty B., 1970: Deacylated transfer rna as an initiator of protein synthesis in higher organisms. Renold, A E 8th International Congress on Biochemistry Symposium 339p 198-200

Goldstein; Walling; Bailey; Chen; Fortner; Klapisch; Phillips; Stewart, 1987: Independent determinations of temperature and ionization balance in a laser-produced plasma by use of L-shell x-ray spectra. Physical Review Letters 58(22): 2300-2303

Schuckit, M.A., 1987: Why don't we diagnose alcoholism in our patients?. Journal of Family Practice 25(3): 225-226

Hollman, J., 1985: Acute occlusion syndrome post PTCA. American Heart Journal 109(6): 1403-1403

NADVORNIK.P.; NEMECEK.S., 1965: Apropos Of Relative Systems In Spinal Cord Stereotaxy. Ceskoslovenska Fysiologie 14: 59-60

Ganderton, D.; Lewis, D.; Davies, R.; Meakin, B.; Brambilla, G.; Church, T., 2002: Modulite: a means of designing the aerosols generated by pressurized metered dose inhalers. Although popular, the pressurized metered dose inhaler generates coarse, fast moving clouds so that the fraction reaching the lung is small. These shortcomings can be redressed by Modulite which permutes the following variables: the non-volatile c...

Whelan C.J.; Johnson M., 1991: Anti inflammatory activity of salmeterol in the guinea pig. European Respiratory Journal 4(SUPPL 13): 166S