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Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction

Genetics 144(4): 1735-1745
Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction
The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.

Accession: 002863758

PMID: 8978059

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