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Identification of some major cytokinins in Phaseolus vulgaris and their distribution


, : Identification of some major cytokinins in Phaseolus vulgaris and their distribution. Physiologia Plantarum 96(1): 77-84

The determination of the various endogenous cytokinins and their distribution among organs is important in understanding their role in growth and development in the intact plant. Cytokinins in young plants of Phaseolus vulgaris were purified by immunoaffinity chromatography and HPLC, and characterised by UV spectra. Zeatin nucleotide (zeatin riboside-5'-monophosphate) and isopentenyladenine nucleotide (isopentenyladenosine-5'-monophosphate) were the most abundant cytokinins in all organs. Their identities were confirmed by GC-MS. The levels of zeatin, zeatin riboside, isopentenyladenine and isopentenyladenosine never exceeded 5% of the nucleotides, as assessed by a methodology that preserves cytokinin nucleotides. Three extraction methods were compared with qualitatively similar results, though differing in their suppression of nucleotidase activity. Cytokinin nucleotide levels were greater in the stems and petioles than in the roots and leaves on a per gram fresh weight basis, and were greater in the stems than in the other organs on a per plant basis. Levels of the zeatin and isopentenyladenine nucleotides were about equal in the stems and leaves, but in the petioles the zeatin nucleotide levels were about twice the level of isopentenyladenine nucleotide, while in the roots they were about half the isopentenyladenine nucleotide level. The importance of considering the cytokinin form is emphasised.

Accession: 002863781

DOI: 10.1111/j.1399-3054.1996.tb00186.x

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Related references

Palmer M.V.; Wong O.C., 1985: Identification of cytokinins from xylem exudate of phaseolus vulgaris. The principal biologically active cytokinins in xylem exudate of young Phaseolus vulgaris L. plants were identified by bioassay, high-performance liquid chromatography, enzymic degradation and combined gas chromatography-mass spectrometry (selecte...

Palmer, M.V.; Wong, O.C., 1985: Identification of Cytokinins from Xylem Exudate of Phaseolus vulgaris L. The principal biologically active cytokinins in xylem exudate of young Phaseolus vulgaris L. plants were identified by bioassay, high-performance liquid chromatography, enzymic degradation and combined gas chromatography-mass spectrometry (selecte...

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Krasnuk, M., 1972: Detection, isolation, and identification of cytokinins from Phaseolus vulgaris L. 'Pinto'. See FCA 25, 3757.

Hammerton, R.D.; Nicander, B.; Tillberg, E., 1994: Light intensity and the organ distribution of cytokinins in Phaseolus vulgaris cv Provider. Biologia Plantarum (Prague) 36(SUPPL ): S25

Palmer, M.V.; Horgan, R.; Wareing, P.F., 1981: Cytokinin metabolism in Phaseolus vulgaris L. : Identification of endogenous cytokinins and metabolism of [8-(14)C]dihydrozeatin in stems of decapitated plants. The major cytokinins in stems of decapitated disbudded bean plants were identified by enzymic degradation,Sephadex LH20 and reversed phase high performance liquid chromatography, and by combined gas chromatography-mass spectrometry as 6-(4-hydroxy...

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