+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

, : Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro. Euphytica 87(3): 165-172

Adventitious shoots were obtained from the diploid watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) cultivars Dixielee, Jubilee II, Mickylee, Minilee, and Royal Sweet by culturing excised cotyledons on shoot regeneration medium for six weeks. Tetraploid and diploid regenerants were identified by counting the number of chloroplasts per guard cell pair from leaves of regenerated plants. Cross fertilization of putative tetraploids with diploid pollinators and the production of triploid seed confirmed the efficacy of this approach. The mean number of chloroplasts for tetraploid regenerants was 19.1 whereas diploids averaged 11.2. These values were similar to tetraploid and diploid plants from seed. Ovary diameter, petal, and anther diameter of male flowers, and leaf length by width ratio were also good indicators of plant ploidy. Progeny obtained from self-fertile tetraploids of 'Mickylee' were crossed with various diploid pollinators to produce triploid hybrid seed. All triploid plants from tissue culture-derived tetraploids produced fruit comparable in quality to fruit produced by currently-available triploid hybrids, demonstrating that in vitro tetraploid induction can be used to produce high quality tetraploid plants for use in triploid hybrid seed production.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863824

DOI: 10.1007/bf00023744

PDF Full Text: Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Abugo, O.; Rifkind, J.M., 1993: Hemoglobin-membrane interactions as probed by nitroblue tetrazolium. Biophysical Journal 64(2 PART 2): A308

Lahiri, A.N.; Audus, L.J., 1961: Growth substances in the roots of Vicia faba. II. The effects of ageing and excision of the main tap-root meristem. The production, distribution and probable functions of the IAA-like AP(ii), the root-growth inhibitor AP(iii) and the root-growth accelerator AP(i) in broad bean seedling roots are discussed on the basis of experimental investigation. -Bedford Col...

Chalumeau, F., 1981: Capture de Termitodius peregrinus Hinton en Guyane et notes diverses (Coleoptera: Scarabaeidae: Aphodiinae: Rhypariini). Bulletin de la Societe Entomologique de Mulhouse, (jan.-mars): 13-16 s

Ramsdell, D.C.; Nelson, J.W.; Myers, R., 1974: An epidemiological study of mummy berry disease of highbush blueberry. Ascospores of Monilinia [Sclerotinia] vaccinii-corymbosi were trapped in a Vaccinium corymbosum field in Mich. from 28 Apr. to 11 May, during which the bushes progressed from green tip to pink bud prebloom stage. Discharge occurred mostly during d...

Crowder, C. M., 1995: A genetic screen in Caenorhabditis elegans for mutations conferring resistance to halothane. Anesthesiology (Hagerstown) 83(3A): A762

Karunakaran, K.; Balakrishnan, S.; Dinakaran, A.M.nicavasaka, 2006: Antiviral chemotherapy for Ranikhet disease. The effectiveness of selective antiviral compound, an appropriate dose for administration and the economic aspect of the use of the drug in treating birds infected with Ranikhet disease was carried out. The drug Amantadine, Ribavirin and their co...

BEER, J., 1960: On differentiation of Bordetella (B.) bronchiseptica with special reference to differentiation from Alcaligenes faecalis. Zentralblatt für Bakteriologie 177: 196-207

Ford, D.H.; Rhines, R.K., 1970: Accumulation of 14C by ventral horn and dorsal root ganglion neurons after intravenous injection of (14C)GABA. Journal of the Neurological Sciences 10(3): 331-337

Balin, V.N.; Iordanishvili, A.K.; Malyshev, V.A.; Fialkovskiĭ, V.V.; Entin, D.A., 1994: D. A. Entin--the founder of Russian military stomatology. Stomatologiia 73(4): 77-80

Pelissonnier H., 1980: Role of underground water of meteoric origin in the formation of mineral deposits. Geologues: ages 49-74. 1980.