+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis

, : Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 91(6-7): 1048-1053

The introgression of rye DNA into the wheat genome was studied using random decamer and specific primers with the polymerase chain reaction (PCR). DNA from paired near-isolines in Chisholm and Arkan backgrounds differing with respect to the presence of a 1 RS.1 BL translocation was amplified with 120 arbitrary sequence primers. Two of the primers (OPR 19 and OPJ07) amplified rye-specific DNA fragments. The OPR19 primer amplified a 1.35-kb fragment that appeared to be specific to the 1 RS.1 BL translocation, based on its presence only in lines carrying the 1 RS. 1 BL translocation. A fragment of the same size was also amplified in 1 RS.1 AL translocation lines. This 1 RS. 1 BL marker locus was designated Ximc 1. The other primer, OPJ07, amplified a 1.2-kb DNA sequence, that was designated Ximc 2, specific to the wheat-rye translocation in various wheat backgrounds. The sequences of the two marker loci were found to be different from each other. The Ximc 1 locus was a low-copy sequence which was also present in Balboa rye genomic DNA. Through the use of specific primers, the presence of the rye-specific marker was confirmed in hexaploid as well as in tetraploid wheat backgrounds. The use of RAPDs for the study of smaller alien introgressions into wheat is discussed.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863827

PMID: 24169995

DOI: 10.1007/BF00223918

PDF Full Text: Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Novikov, N. E., 1986: Regeneration of oak stands on the banks of the hydrographic system. Trials were started in the Orel region in 1975 in regenerating 50-yr-old stands of oak on SE slopes with eroded soils. The methods compared involved: (a) clear felling strips 30 m wide for coppice regeneration; (b) cultivating strips 0.5 m wide to...

Ma, L.; Hunt, A.G., 2015: A 3' RACE protocol to confirm polyadenylation sites. A routine procedure in the study of polyadenylation involves the determinations of the junctions of the mRNA body and the poly(A) tail (the poly(A) site). This is typically accomplished by selectively amplifying the 3'-ends of cDNAs (3'-...

Chavez R., 1992: Effects of superior ovarian nerve son section on each day of estrous cycle on the compensatory ovarian hypertrophy coh in the hemiovariectomized adult rat. Biology of Reproduction 46(SUPPL 1): 114

Shaw, P., J.; Ince, P., G., 1994: A quantitative autoradiographic study of (3H)kainate binding sites in the normal human spinal cord, brainstem and motor cortex. The quantitative autoradiographic distribution of the kainate subtype of non-NMDA receptor in the normal human motor cortex, brainstem and spinal cord has been investigated using (3H)kainate. In the motor cortex specific (3H)kainate binding was pr...

Hathaway, D. E., 1978: The U.S. experience as a dairy doner country. The 36 main dairy producing countries produce approx. 90% of the world's milk. Consumption has not kept pace with production, and surpluses of dairy products (in the US particularly butter and dried skim milk) have resulted. A proportion of t...

NEIDHARDT.F.C.; EIDLIC.L., 1963: Characterization of the RNA formed under conditions of relaxed amino acid control in Escherichia coli. Biochimica et Biophysica Acta 68: 380-388

Rung, J.; Tricot, B.; Copin, A.; Culot, M., 2002: Database "SIGMA Pro" information system and bibliographical management of active substances of plant protection products and biocides. In order to satisfy its obligations as stipulated in European legislative, in 1998, the Management of Surface Waters in the Walloon Region modified its monitoring of plant protection products in surface waters in co-operation with the Regional Phy...

Chaly, T.; Diksic, M., 1986: High yield synthesis of 6-[18F]fluoro-L-dopa by regioselective fluorination of protected L-dopa with [18F]acetylhypofluorite. Regioselective fluorination of a completely protected phosgene derivative of 3,4-dihydroxy-phenyl-L-alanine (5-(benzyl-3',4'-carbonate)-oxazolidine-2,5-dione) with gaseous 18F-labeled acetylhypofluorite and [18F]F2 in acetonitrile is des...

Monteiro, L.; Coelho, T.; Stocker, A., 1987: Neuro-cysticercosis: a parasitic disease frequent in Portugal. 138 cases diagnosed by computed tomography of the brain. Of about 17 000 brain scans carried out at the Santo Antonio General Hospital, Porto, Portugal, from November 1982 to June 1986. 138 showed typical images of cerebral cysticerciasis. 41 (30%) of the patients were asymptomatic and were discovered d...

Jiang, J.; He, Y.; Jiang, R., 2010: Ultrastructural changes of penile cavernous tissue in multiple sclerotic rats. Multiple sclerosis (MS) is one of the important risk factors resulting in erectile dysfunction (ED). The ultrastructure of corpus cavernous of the penis have an important role in the mechanism of erection. It is suggested that different medical co...