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Identification of the major starch synthase in the soluble fraction of potato tubers


, : Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell 8(7): 1121-1135

The major isoform of starch synthase from the soluble fraction of developing potato tubers has been purified and used to prepare an antibody and isolate a cDNA. The protein is 140 kD, and it is distinctly different in predicted primary amino acid sequence from other isoforms of the enzyme thus far described. Immunoinhibition and Immunoblotting experiments and analysis of tubers in which activity of the isoform was reduced through expression of antisense mRNA revealed that the isoform accounts for approximately 80% of the activity in the soluble fraction of the tuber and that it is also bound to starch granules. Severe reductions in activity had no discernible effect on starch content or amylose-to- amylopectin ratio of starch in tubers. However, they caused a profound change in the morphology of starch granules, indicative of important underlying changes in the structure of starch polymers within the granule.


Accession: 002863879

PMID: 8768372

DOI: 10.1105/tpc.8.7.1121

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Related references

Ponstein A.S.; Oosterhaven K.; Feenstra W.J.; Witholt B., 1991: Starch synthesis in potato tubers identification of the in vitro and in vivo acceptor molecules of soluble starch synthase activity. A soluble starch synthase preparation obtained from potato tubers (Solanum tuberosum L.) was used to identify the acceptor molecules of the enzyme in vitro. Maltose was the shortest effective primer molecule, and several straight and branched gluc...

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