EurekaMag.com logo
+ Translate

Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases


, : Identification of the root-knot nematode, Meloidogyne incognita, using monoclonal antibodies raised to non-specific esterases. Physiological & Molecular Plant Pathology 49(2): 79-88

The esterase isozyme from Meloidogyne incognita with pI apprx 4.95 was purified using a Rotofor electrophoresis system. The purified enzyme was then used to raise monoclonal antibodies. Hybridoma cell lines producing antibodies were cloned and screened against the purified esterase in an enzyme-linked immunosorbent assay (ELISA). A selected monoclonal antibody was then tested by western blot analysis of crude soluble protein extracts of all the major species of Meloidogyne separated by native and SDS-PAGE electrophoresis. Although the antibody was cross-reactive in western blots of denatured proteins in the presence of SDS and in ELISA, it was not cross-reactive in western blots of native protein. The results demonstrate that a monoclonal antibody to a diagnostic non-specific esterase enables M. incognita to be discriminated from M. javanica without the need to separate the esterases by electrophoresis first. This is an approach which could be adopted in a number of situations where organisms can be identified by a diagnostic protein which has already been identified by electrophoresis.

(PDF 0-2 workdays service)

Accession: 002863908

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Farooqi, T.N.A.; Dasgupta, D.R., 1978: Esterases activity from the root knot nematode meloidogyne incognita. Esterases activity from the root-knot nematode, M. incognita was investigated with substrates, O-NPA [nitrophenylacetate], IPA [indophenylacetate] and Ach [acetylcholine]. Activity of the enzyme was more in larval tissues homogenates than in egg m...

Anonymous, 2013: Protective effect of Dryopteris crassirhizoma extracts in the control of the root-knot nematode Meloidogyne incognitaol of the root-knot nematode Meloidogyne incognita. Root-knot nematodes (Meloidogyne spp.) are among the most damaging soilborne plant parasites and are responsible for large economic losses in a wide variety of crops worldwide. More recently attention has increased on the use of phytochemicals as...

Fassullotis, G., 1973: Susceptibility of eggplant, Solanum melongena, to root-knot nematode, Meloidogyne, to root-knot nematode, Meloidogyne incognita. Plant Disease Reporter, 577: 606-608

Averre, Cw, 1982: Control of root-knot nematode on processing tomatoes, 1981 Tomato (Lycopersicon esculentum L. Campbells C-37), root-knot nematode; Meloidogyne incognita. Fungicide and nematicide tests results American Phytopathological Society7(37): 202

Fukudome, N.; Sakasegawa, Y., 1972: Interaction between root-knot nematode and other diseases. II. Influence of the root-knot nematode (Meloidogyne incognita) on the occurrence of Granville wilt on tobacco. In order to clarify the relationships between Meloidogyne incognita and the occurrence of Granville wilt of tobacco caused by Pseudomonas solanacearum, a series of experiments was conducted. A simultaneous inoculation of the nematode and bacteria...

Tesarova, B.; Zouhar, M.; Rysanek, P., 2003: Development of PCR for specific determination of root-knot nematode Meloidogyne incognita. PCR primers designed from the gene sequence for the SEC 1 oesophageal gland protein were used to specifically detect and differentiate the root-knot nematode Meloidogyne incognita from other species from the genus Meloidogyne. Amplification produc...

Kantharaju, V.; Krishnappa, K.; Reddy, B.M.R., 2006: Identification of virulent isolates of root-knot nematode, Meloidogyne incognita on tomato. The 25 indigenous isolates of root-knot nematode, Meloidogyne incognita collected from different agro climatic zones and crops of Karnataka were tested on tomato cv. Pusa Ruby. Among these, GKVK coleus isolate, Mandya tomato isolate, Davanagere b...

Subramanian, P.; Choi, I-Chan.; Mani, V.; Park, J.; Subramaniyam, S.; Choi, K-Hyun.; Sim, J-Soo.; Lee, C-Muk.; Koo, J.Choon.; Hahn, B-Soo., 2016: Stage-Wise Identification and Analysis of miRNA from Root-Knot Nematode Meloidogyne incognita. NlmCategory="UNASSIGNED">In this study, we investigated global changes in miRNAs of <i>Meloidogyne incognita</i> throughout its life cycle. Small RNA sequencing resulted in approximately 62, 38, 38, 35, and 39 Mb reads in...

Castro, C.E.; Belser, N.O.; McKinney, H.E.; Thomason, I.J., 1990: Strong repellency of the root knot nematode,Meloidogyne incognita by specific inorganic ions. Simple inorganic salts of the ions K(+), NH 4 (+) , Cs(+), NO 3 (-) , and Cl(-) are strongly repellent to infective second-stage larvae of the root knot nematode,Meloidogyne incognita. Some of these salts are known to be beneficial to plant growth...

Castro, C.; Belser, N.; Mckinney, H.; Thomason, I., 1990: Strong repellency of the root knot nematode, Meloidogyne incognita by specific inorganic ions. Simple inorganic salts of the ions K+, NH4+, Cs+, NO3(-), and Cl(-) are strongly repellent to infective second-stage larvae of the root knot nematode, Meloidogyne incogrzita. Some of these salts are known to be beneficial to plant growth. The resu...