EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport






Proceedings of the National Academy of Sciences of the United States of America3, 93(15): 8145-8150

Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.

Accession: 002863944

PMID: 8755618

DOI: 10.2307/40178

Download PDF Full Text: Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport



Related references

Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis. Genetics 152(1): 401-412, 1999

Arabidopsis dwarf mutants define eight genes involved in brassinosteroid biosynthesis and signal transduction. Plant Biology (Rockville) : 10, 1998

Patatin like phospholipase A genes from Arabidopsis are involved in auxin signal transduction and their transcription responds to biotic and abiotic signals. Plant Biology (Rockville) : 176, 2002

Identification of new genes involved in ras-mediated signal transduction in C elegans. Journal of Cellular Biochemistry Supplement 0(18B): 260, 1994

Identification of signal transduction pathways leading to the expression of Arabidopsis thaliana defense genes. Advances in molecular genetics of plant microbe interactions Volume I Proceedings of the 5th International Symposium, Interlaken, Switzerland, 9-14 September 1990: 357-364, 1991

Identification of genes involved in two-component signal transduction in Burkholderia pseudomallei. Biochemical Society Transactions 28(5): A282, October, 2000

Identification and characterization of genes involved in signal transduction in the nervous system. Journal of Cellular Biochemistry Supplement (15 PART B): 203, 1991

Phylogenetic analysis of plant type one protein phosphatases (PP1) and identification of transferred DNA (T-DNA) insertions in an Arabidopsis PP1 gene. Current topics in plant biochemistry and physiology: proceedings of the Plant Biochemistry and Physiology Symposium held at the University of Missouri Columbia: 6th) 115, 1997

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9(5): 759-771, 1997

Nonselective block by La3+ of Arabidopsis ion channels involved in signal transduction. Journal of Membrane Biology 162(1): 81-90, 1998