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Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

, : Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. Proceedings of the National Academy of Sciences of the United States of America3, 93(15): 8145-8150

The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.

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Accession: 002863944

DOI: 10.2307/40178

PDF Full Text: Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

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Other references

Jamieson, A.T.; Webb, D.R., 1977: Early changes in splenic DNA synthesis induced by thymic-dependent and thymic-independent antigens. Clinical Immunology and Immunopathology 7(2): 219-229

Tormo-Badia, N.; Håkansson, Å.; Vasudevan, K.; Molin, G.; Ahrné, S.; Cilio, C.M., 2014: Antibiotic treatment of pregnant non-obese diabetic mice leads to altered gut microbiota and intestinal immunological changes in the offspring. The intestinal microbiota is important for tolerance induction through mucosal immunological responses. The composition of the gut microbiota of an infant is affected by environmental factors such as diet, disease and antibiotic treatment. However...

Greilhuber, J., 1979: Evolutionary changes of DNA and heterochromatin amounts in the Scilla bifolia group (Liliaceae). Plant systematics and evolution: uppl2) 263-280

Cruz Reyes A., 1973: Cestodes of fishes part 1 re description of the subgenus otobothrium pseudotobothrium and of the species otobothrium dipsacum. Anales del Instituto de Biologia Universidad Nacional Autonoma de Mexico Serie Zoologia 44(1): 25-34

Beeson Marvin H.; Tolan Terry L., 1997: Geologic news from the Westside Tunnel. Annual Meeting - Association of Engineering Geologists 40: 84

Matsuo, R.; Misawa, K.; Ito, E., 2008: Genomic structure of nitric oxide synthase in the terrestrial slug is highly conserved. Nitric oxide (NO) is a key molecule in olfactory information processing across animal species. To gain insight into the genetic basis of NO generation, we investigated the genomic structure of nitric oxide synthase (NOS) in the terrestrial slug L...

Matijevic, S.; Wiebe, S.; Punambolam, R.; Cervinka, A.; Alikhan, Q., 2003: Outpatient investigations predict surgical decisions in patients with temporal lobe epilepsy and mesial temporal sclerosis. Epilepsia 44(Supplement 9): 49

Bergstrom, D.; Bergsten, U.; Nordfjell, T.; Lundmark, T., 2007: Simulation of geometric thinning systems and their time requirements for young forests. In Fennoscandia, large areas that have not been subjected to pre-commercial thinning (PUT), and thus support dense stands, are becoming suitable for harvesting biomass. However, efficient systems for harvesting biomass from young stands have not...

Pareto A., 1980: Improved staining procedure for cell nuclei and nucleoli of basidiomycetes with giemsa solution. Mikrokosmos 69(7): 239

Schmah, T.; Marwan, N.; Thomsen, J.Skovhus.; Saparin, P., 2011: Long range node-strut analysis of trabecular bone microarchitecture. We present a new morphometric measure of trabecular bone microarchitecture, called mean node strength (NdStr), which is part of a newly developed approach called long range node-strut analysis. Our general aim is to describe and quantify the appar...