+ Translate

Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

, : Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. Proceedings of the National Academy of Sciences of the United States of America3, 93(15): 8145-8150

The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863944

PMID: 8755618

DOI: 10.2307/40178

Download PDF Full Text: Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Greenberg, M.; Lowrie, K., 2012: Daniel Kahneman: how we think and choose. Risk Analysis 32(7): 1113-1116

Sepúlveda, B.; Aubanel, M.; Landa, L.; Velázquez, G., 1972: Advances in the counter-immuno-electrophoresis technic for the serologic study of amebiasis. Archivos de Investigacion Medica 2: Suppl 2:363-70

Meyer, F.M.nteuffel, U.V.n, 1973: Ralgro for the intensive fattening of young cattle. Kraftfutter 56 (9) 417-418

Lehrer, M.; Bischof, S., 1995: Detection of model flowers by honeybees: The role of chromatic and achromatic contrast. Honey bees [Apis mellifera] were trained to collect sugar solution from an experimental apparatus which incorporated a white or yellow paper background and a rewarded channel which carried a disk of a contrasting colour - black or grey to contrast...

Lee, C.Q.; Shey, Z.; Cobb, C.M., 1995: Microscopic appearance of enamel white-spot lesions after acid etching. Ultrastructural changes in surface characteristics of enamel white-spot lesions were compared with changes in the adjacent clinically sound enamel after they were etched with 30% phosphoric acid. Ten human permanent first molars exhibiting natural...

Sorensen K.; Sorensen A.M.; Nielsen J., 1981: Psychological and social development of adolescents with klinefelters syndrome. Clinical Genetics 20(5): 395

Soffel H.C.; Foerster H.G., 1979: Polar wander path of central Iran and its interpretation. International Union of Geodesy and Geophysics, General Assembly 17): 515

Russell R.D.; Butler K.E.; Kepic A.W.; Maxwell M., 1997: Seismoelectric exploration. Leading Edge (Tulsa, OK) 16(11): 1611-1615

Iannotti, J.P., 2006: Developing a management algorithm for persistent shoulder pain. American Journal of Orthopedics 34(12 Suppl): 4-4

Moore, N. C., 1977: Medazepam and the driving ability of anxious patients. A double blind crossover trial of medazepam was carried out in 14 anxious hospital patients. The mean self-adjusted dosage was 16.5 mg daily. The active drug was no more effective than placebo in relieving anxiety, which was rated both clinically...