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Identification of vitamins A, D and E by particle beam liquid chromatography-mass spectrometry


, : Identification of vitamins A, D and E by particle beam liquid chromatography-mass spectrometry. Fresenius' Journal of Analytical Chemistry 351(8): 768-776

Particle beam (PB) high performance liquid chromatography (HPLC)-mass spectrometry (MS) has been studied to investigate its suitability for the analysis of fat-soluble vitamins, as an example of thermally labile substances. The PB LC-MS system has been used to detect and identify vitamins A, D and E in foods and multi-vitamin preparations. The qualitative performance of the PB interface has been evaluated by an examination of the working parameters. A rapid elution of the vitamins was obtained on a C8 reversed-phase column using an aqueous methanol mobile phase. Analyses have been carried out using both, electron (EI) and chemical (CI) ionization. Positive and negative-ion CI spectra of the compounds are discussed. When PB-MS conditions are optimized, the detection limits for the fat-soluble vitamins tested have been typically in the 0.6–25 ng range when using the selected-ion monitoring technique.

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Accession: 002863980

DOI: 10.1007/bf00323634

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White, W.A.; Kyriazis, M.K., 1968: Effects of waste effluents on the plasticity of earth materials. Earth materials were treated with soap, detergents, starch, water softeners, and fabric softeners, and the Atterberg plastic limits and activity values were determined.

Garrido, A.; Busto, T. del; Tarancon, J., 1982: Effect of some pesticides in the laboratory on the immature stages of Aleurothrixus floccosus Mask. I. Egg. In laboratory studies in Spain in which 24 insecticides, 2 acaricides and a detergent were tested for the control of the eggs of the citrus pest Aleurothrixus floccosus (Mask.), only 5 of the products gave more than 50% control: 0.25% butocarboxim...

Kaplan, B., L.F.; Norell, H.; Callender, G., G.; Ohlum, T.; Kiessling, R.; Nishimura, M., I., 2006: Interferon-gamma renders tumors that express low levels of Her-2/neu sensitive to cytotoxic T cells. Her-2/neu is a tumor-associated antigen that has been targeted with both antibodies and cytotoxic T lymphocytes (CTL). Despite the isolation of Her-2/neu-reactive CTL in vaccinated patients, their therapeutic use has been limited by the observatio...

Roth, H.T.; Marshall, R.T.; Schmidt, F.F., 1968: Solubilization of Cottage cheese curd by alkaline wash waters and its causes. Cottage cheese was manufactured and washed twice in each of several water-mineral mixtures adjusted to pH 4.8, 5.0, 7.0, 9.0 or 11.0. Curd solubilization was detected only in water adjusted to pH 11.0. However, water adjusted to pH 11.0 and contai...

WANGENSTEEN, O.H., 1953: The worth of a modern hospital to a community. Journal-Lancet 73(7): 297-9; Passim

Malva, J.; Carvalho, A.; Carvalho, C., 1996: Identification of a presynaptic kainate receptor which modulates the exocytotic release of glutamate in rat hippocampal CA3 synaptosomes. FASEB Journal 10(3): A154

Balaji, S.; Indira Kalyanasundaram, 2002: Antimicrobial activity of some indigenous fumigants. Efficacy of certain indigenous fumigants as antimicrobial agents was evaluated in the present work. Against three test organisms viz., Bacillus subtilis, Aspergillus niger, and A. flavus, the efficacy of fumes of incense, Garlic, Turmeric, Neem (A...

Chen, H.Z., 1981: Heart diseases seen in Shanghai and the change of their incidences in the past 32 years--with an analysis of the data of 15,696 cases. Zhonghua Nei Ke Za Zhi 20(1): 6-10

Morris, A.E.; Lutman, M.E.; Yardley, L., 2009: Measuring outcome from vestibular rehabilitation, part II: refinement and validation of a new self-report measure. A prototype self-report measure of vestibular rehabilitation outcome is described in a previous paper. The objectives of the present work were to identify the most useful items and assess their psychometric properties. Stage 1: One hundred fifty-f...

Bernardini, S.; Cianfarani, S.; Spagnoli, A.; Annicchiarico-Petruzzelli, M.; Melino, G.; Massoud, R.; Boscherini, B.; Finazzi-Agró, A.; Rosenfeld, R.G.; Federici, G., 1994: Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells. Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the pres...