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Improvement of frost tolerance in winter barley by in vitro-selection of hydroxyproline-resistant lines derived from anther cultures



Improvement of frost tolerance in winter barley by in vitro-selection of hydroxyproline-resistant lines derived from anther cultures



Nordisk Jordbruksforskning 78(2): 106



Embryogenic callus from anther culture of the variety Igri was plated on solid L3 medium with hydroxyproline (Hyp). After 3 months' subculture, Hyp-resistant callus was transferred to regeneration medium. The 33 Hyp-resistant plant regenerated were cold hardened at 2 degrees C and after 0, 14 and 28 days' hardening the frost tolerance of leaves was measured using the conductivity technique. The Hyp-resistant regenerants were significantly more frost tolerant than the controls.

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Accession: 002866617

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