Section 3
Chapter 2,868

In vitro propagation of Higo chrysanthemum (Dendranthema x grandiflorum (Ramat.) Kitam.)

Roxas, N.J.L.; Tashiro, Y.; Miyazaki, S.; Isshiki, S.; Takeshita, A.

Bulletin of the Faculty of Agriculture, Saga University 80: 57-67


ISSN/ISBN: 0581-2801
Accession: 002867828

Download citation:  

Higo chrysanthemum (Dendranthema grandiflorum [D. morifolium]) is a unique group of chrysanthemums raised and cultivated in the Kumamoto area of Japan for over 200 years. To develop a safe and economical method of propagation through tissue culture, different culture conditions were tested using 29 cultivars. Efficient in vitro propagation was achieved when nodal segments were cultured in hormone-free MS medium at 25 degrees C. In vitro rooting was not essential for the transfer of regenerated shoots to the nursery bed, as vigorous growth and rapid root formation was demonstrated by cuttings from the regenerated shoots. No somaclonal variation was detected in regenerated plantlets.

PDF emailed within 1 workday: $29.90