Section 3
Chapter 2,868

In vitro propagation of Rauwolfia micrantha, a rare medicinal plant

Sudha, C.; Seeni, S.

Plant cell tissue and organ culture 44(3): 243-248


ISSN/ISBN: 0167-6857
DOI: 10.1007/bf00048530
Accession: 002867843

Shoot tip and single node explants from young shoots of 1-year old flowering plants of Rauwolfia micrantha Hook. f. were cultured on Murashige & Skoog (MS) medium variously supplemented with 6 - benzyladenine (BA) and alpha - naphthaleneacetic acid (NAA). A combination of 13.2 micromolar BA and 2.68 micromolar NAA induced high frequency (77%) formation of up to 3 shoots from each node in 8 weeks. The regeneration of shoot tips from the field-grown plants and in vitro shoots placed horizontally differed. Repeated subculturing of the shoot tips and single nodes at 6-week intervals for over a year in combination of 4.4 micromolar BA and 0.27 micromolar NAA enabled mass multiplication of shoots without any evidence of decline. Rooting of the excised shoots on medium containing 2.6 micromolar NAA was preceded by callus formation. The rooted plants were removed off the callus, hardened off and 80% established in pots. Micropropagated plants displayed uniform morphological, growth, flowering, fruiting and seed germination characteristics.

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