+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Infection and cross-protection studies of winter dysentery and calf diarrhea bovine coronavirus strains in colostrum-deprived and gnotobiotic calves

Infection and cross-protection studies of winter dysentery and calf diarrhea bovine coronavirus strains in colostrum-deprived and gnotobiotic calves

American Journal of Veterinary Research 57(1): 48-53

Objective-To investigate in vitro antigenic relations, in vivo cross-protection, and isotype antibody responses to a winter dysentery (WD) and calf diarrhea strain of bovine coronavirus (BCV). Design and Animals-Gnotobiotic and colostrum-deprived calves were inoculated oronasally with a WD (DBA) or a calf diarrhea (DB2) BCV, and were challenge exposed with the heterologous BCV. Procedure-Nasal swab and feces specimens and blood samples were collected. Fecal and nasal specimens were assayed for BCV shedding by antigen-capture ELISA or immune electron microscopy. Bovine coronavirus antigens were detected in nasal epithelial cells by immunofluorescence. Antibody titers to BCV in serum were assayed by virus neutralization (VN), and BCV antibody isotype titers in feces and sera were quantitated by ELISA. Results-All calves developed diarrhea and shed BCV nasally and in feces, then recovered and were protected from BCV-associated diarrhea after challenge exposure with the heterologous BCV. After challenge exposure with either strain, fecal shedding of DBA was detected in 1 of 4 calves and nasal shedding of DB2 was detected in 2 of 4 calves. Immunoglobulin M was the principal coproantibody to BCV early, followed predominantly by IgA. Immunoglobulin G-1 coproantibody titers to BCV were low, but increased after challenge exposure. Immunoglobulin G-1 antibodies were predominant in serum. After challenge exposure, all serum antibody isotype titers increased except IgG-2. The VN antibody responses paralleled serum IgG-1 antibody responses. Conclusions and Clinical Relevance-Immunoglobulin A coproantibodies at challenge exposure were associated with protection against diarrhea. Nasal shedding of BCV after challenge exposure confirmed field data documenting reinfection of the respiratory tract of cattle, suggesting that, in closed herds, respiratory tract infections constitute a source of BCV transmission to cows (WD) or young calves.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 002870302

Download citation: RISBibTeXText

PMID: 8720237

Related references

Cross-protection studies between respiratory and calf diarrhea and winter dysentery coronavirus strains in calves and RT-PCR and nested PCR for their detection. Archives of Virology 146(12): 2401-2419, 2001

Cross-protection against a human enteric coronavirus and a virulent bovine enteric coronavirus in gnotobiotic calves. Journal of Virology 80(24): 12350-6, 2006

Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or colostrum antibody powder. Veterinary Microbiology 58(2-4): 105-111, 1997

Detection of bovine coronavirus and type A rotavirus in neonatal calf diarrhea and winter dysentery of cattle in Quebec: evaluation of three diagnostic methods. Canadian Veterinary Journal 35(3): 163-169, 1994

Experimental reproduction of diarrhoeas with bovine coronavirus in newborn calves deprived of colostrum. Revista de Salud Animal 27(3): 171-175, 2005

The nutritive value of colostrum for the calf. 14. Further studies on the effect of antibiotics on the performance of colostrum-deprived calves. British Journal of Nutrition 12(2): 203-215, 1958

Experimental infection of colostrum-deprived calves with bovine viral diarrhea virus type 1a isolated from free-ranging white-tailed deer (Odocoileus virginianus). Canadian Journal of Veterinary Research 75(1): 65-68, 2011

Bovine epizootic diarrhea resembling winter dysentery caused by bovine coronavirus. JARQ Japan Agricultural Research Quarterly 17(3): 185-190, 1983

The experimental production of diarrhea in colostrum deprived axenic and gnotoxenic calves with entero pathogenic escherichia coli rotavirus coronavirus and in a combined infection of rotavirus and escherichia coli. Annales de Recherches Veterinaires 9(3): 433-440, 1978

Protection studies on winter dysentery caused by bovine coronavirus in cattle using antigens prepared from infected cell lysates. Canadian Journal of Veterinary Research 64(2): 138-140, 2000

Failure to reproduce diarrhea in colostrum deprived new born calves infected with cell culture bovine rotavirus. Annales de Medecine Veterinaire 126(1): 59-62, 1982

Effect of infection with bovine viral diarrhea virus alone, bovine rotavirus alone, or concurrent infection with both on enteric disease in gnotobiotic neonatal calves. American Journal of Veterinary Research 63(8): 1179-1186, 2002

Dual enteric and respiratory tropisms of winter dysentery bovine coronavirus in calves. Archives of Virology 152(10): 1885-1900, 2007

Protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge. Journal of Infectious Diseases 148(6): 1061-1068, 1983

Isotype-specific antibody responses to bovine coronavirus structural proteins in serum, feces, and mucosal secretions from experimentally challenge-exposed colostrum-deprived calves. American Journal of Veterinary Research 52(5): 692-699, 1991