EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification






Plant Molecular Biology 34(6): 843-854

Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification

We have previously shown that intact plants and cultured plant cells can metabolize and detoxify formaldehyde through the action of a glutathione-dependent formaldehyde dehydrogenase (FDH), followed by C-1 metabolism of the initial metabolite (formic acid). The cloning and heterologous expression of a cDNA for the glutathione-dependent formaldehyde dehydrogenase from Zea mays L. is now described. The functional expression of the maize cDNA in Escherichia coli proved that the cloned enzyme catalyses the NAD(+)- and glutathione (GSH)-dependent oxidation of formaldehyde. The deduced amino acid sequence of 41 kDa was on average 65% identical with class III alcohol dehydrogenase from animals and less than 60% identical with conventional plant alcohol dehydrogenases (ADH) utilizing ethanol. Genomic analysis suggested the existence of a single gene for this cDNA. Phylogenetic analysis supports the convergent evolution of ethanol-consuming ADHs in animals and plants from formaldehyde-detoxifying ancestors. The high structural conservation of present-day glutathione-dependent FDH in microorganisms, plants and animals is consistent with a universal importance of these detoxifying enzymes.

Accession: 002889083

PMID: 9290637

DOI: 10.1023/a:1005872222490

Download PDF Full Text: Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification



Related references

Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Plant Physiology 132(4): 2248-2255, 2003

Structure of the Drosophila melanogaster glutathione-dependent formaldehyde dehydrogenase/octanol dehydrogenase gene (class III alcohol dehydrogenase): Evolutionary pathway of the alcohol dehydrogenase genes. European Journal of Biochemistry 225(3): 985-993, 1994

Maize glutathione-dependent formaldehyde dehydrogenase: protein sequence and catalytic properties. Planta 208(1): 12-18, 1999

Pathways for transcriptional activation of a glutathione-dependent formaldehyde dehydrogenase gene. Journal of Molecular Biology 280(5): 775-784, 1998

Characterization of the functional gene encoding mouse class III alcohol dehydrogenase (glutathione-dependent formaldehyde dehydrogenase) and an unexpressed processed pseudogene with an intact open reading frame. European Journal of Biochemistry 237(2): 496-504, 1996

A glutathione-dependent formaldehyde dehydrogenase gene of symbiotic cyanobacterium Anabaena azollae. Symbiosis Rehovot 30(1): 41-55, 2001

Overexpression of the formaldehyde dehydrogenase gene from Brevibacillus brevis to enhance formaldehyde tolerance and detoxification of tobacco. Applied Biochemistry and Biotechnology 169(1): 170-180, 2013

Function of a glutathione-dependent formaldehyde dehydrogenase in Rhodobacter sphaeroides formaldehyde oxidation and assimilation. Biochemistry 37(2): 530-537, 1998

Molecular characterization of the glutathione-dependent formaldehyde dehydrogenase gene FLD1 from the methylotrophic yeast Pichia methanolica. Yeast 21(5): 445-453, 2004

Characterisation of the glutathione-dependent formaldehyde detoxification pathway in Arabidopsis, wheat and rice. Plant Biology (Rockville) : 139, 2002