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Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification


, : Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification. Plant Molecular Biology 34(6): 843-854

We have previously shown that intact plants and cultured plant cells can metabolize and detoxify formaldehyde through the action of a glutathione-dependent formaldehyde dehydrogenase (FDH), followed by C-1 metabolism of the initial metabolite (formic acid). The cloning and heterologous expression of a cDNA for the glutathion-dependent formaldehyde dehydrogenase from Zea mays L. is now described. The functional expression of the maize cDNA in Escherichia coli proved that the cloned enzyme catalyses the NAD+- and glutathione (GSH)-dependent oxidation of formaldehyde. The deduced amino acid sequence of 41 kDa was on average 65% identical with class III alcohol dehydrogenases from animals and less than 60% identical with conventional plant alcohol dehydrogenases (ADH) utilizing ethanol. Genomic analysis suggested the existence of a single gene for this cDNA. Phylogenetic analysis supports the convergent evolution of ethanol-consuming ADHs in animals and plants from formaldehyde-detoxifying ancestors. The high structural conservation of present-day glutathione-dependent FDH in microorganisms, plants and animals is consistent with a universal importance of these detoxifying enzymes.

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Accession: 002889083

DOI: 10.1023/a:1005872222490

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Other references

Ordman A.M.; Kirschenbaum D.S., 1986: Bulimia assessment of eating psychological adjustment and familial characteristics. The eating attitudes and behavior, psychological functioning, and family dynamics of 25 women with bulimia (binge-purge syndrome) were examined by comparing their responses on self-report measures to those of a normal control group. As expected, t...

Pols, H.A.P.; Uitterlinden, A.G., 2003: Genetic polymorphisms and clinical practice: the example of osteoporosis. Over the past decades epidemiological research of so-called "complex" diseases, such as diabetes, osteoporosis, cancer, and cardiovascular disease, has identified a number of risk-factors for these common age-related disorders. Examples...

Amer, F.I.; Elshabka, H.A., 1978: Studies on the reproductive organs of the Colubrid snakes, Psammophis sibilans and Spalerosophis diadema, I. the male organs. Testes of Psammophis sibilans are reduced in size and in weight in summer (inactive period), increase gradually during autumn and winter and reach their maximum at early spring (breeding season). Those of Spalerosophis diadema are smallest in wint...

Armour J.A.; Klassen G.A.; Hodgson E.; Mcgill M., 1980: The control of coronary venous flow. Federation Proceedings 39(3): ABSTRACT 1402

Dixon, W.E., 1907: The action of alcohol on the circulation. Journal of Physiology 35(4): 346-366

Chullasorn, Supawadee, 2010: Two new species of Paramphiascella Lang Copepoda Harpacticoida Miraciidae from a brackish water pond in Thailand. Two new species of the harpacticoid genus Paramphiascella Lang, 1944 were collected from a brackish water pond located at the Coastal Fisheries Research Development Center, Department of Fisheries, Samut Sakhon province, Thailand, and are describe...

Datz, F.L.; Christian, P.E.; Hutson, W.R.; Moore, J.G.; Morton, K.A., 1991: Physiological and pharmacological interventions in radionuclide imaging of the tubular gastrointestinal tract. Radionuclide studies of the gastrointestinal tract (GI) are used to measure GI function and to detect anatomic lesions such as Meckel's diverticuli. A variety of physiological and pharmacological interventions have been applied to both types...

Wang, D.; Liu, N.; Wang, L.; Ni, L.; Zhang, Z., 2013: Characterization of a novel HLA-C*07 allele, HLA-C*07:56:01. A novel HLA-C allele, C*07:56:01, is the close matching allele C*07:02:01:01 by one nucleotide substitution in exon 2, at position 343 G>A.

Veselý, L., 1965: Aplasia of the inferior rectus oculi. Ceskoslovenska Oftalmologie 21(6): 477-478

Kozai, T.; Kino, S.; Jeong, B.R.; Hayashi, M.; Kinowaki, M.; Ochiai, M.; Mori, K., 1992: A sideward lighting system using diffusive optical fibres for production of vigorous micropropagated plantlets. A side lighting system (SLS) using diffusive optical fibres was developed for increased energy and space efficiency and for the elimination of thermal radiation problems. Plantlets of potato were grown on MS medium containing 0 and 20 g/litre sucr...