+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Maize seedlings show cell-specific responses to heat shock as revealed by expression of RNA and protein

Developmental Genetics 18(3): 244-253

Maize seedlings show cell-specific responses to heat shock as revealed by expression of RNA and protein

The cellular localization of heat-shock proteins has been described in a number of experimental animal systems but is not well defined in plant systems. Sense and antisense RNA transcripts from the open reading frame (ORF) of 18-kDa maize heat-shock protein genes were employed in in situ hybridizations of inbred Oh43 radicles and plumules to reveal the locations of their mRNAs. Localization of the specific mRNAs to the younger meristematic cells of the root-tips and shoot-tips and also to the densely cytoplasmic cells of the vasculature was observed routinely. The ORF of one of our 18-kDa genes was cloned into an expression vector, and the 161-amino acid polypeptide was used to raise antibodies. Using a Fast Red procedure, the cellular positions of the heat-shock protein-antibody conjugates were observed in sections similar to those employed in the antisense RNA in situ hybridizations. The localization of the antibody appears to parallel closely the patterns of distribution of the mRNAs.

Accession: 002889115

PMID: 8631158

DOI: 10.1002/(SICI)1520-6408(1996)18:3<244::AID-DVG5>3.0.CO;2-9

Download PDF Full Text: Maize seedlings show cell-specific responses to heat shock as revealed by expression of RNA and protein

Related references

Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under abiotic stresses in rice. Plant Science 176(4): 583-590, 2009

The maize heat shock factor-binding protein paralogs EMP2 and HSBP2 interact non-redundantly with specific heat shock factors. Planta 224(1): 42-52, 2005

T cell responses to heat-shock protein 60: differential responses by CD4+ T cell subsets according to their expression of CD45 isotypes. Journal of Immunology 162(2): 704-710, 1999

Characterization of two maize HSP90 heat shock protein genes: expression during heat shock, embryogenesis, and pollen development. Developmental Genetics 14(1): 27-41, 1993

Expression of heat shock factor and heat shock protein 70 genes during maize pollen development. Plant molecular biology 29(4): 841-856, 1995

Heat-shock protein-specific T-cell responses in various stages of bovine paratuberculosis. Veterinary immunology and immunopathology, 70(1-2): 105-115, 1999

On the model of feedback regulation of heat shock gene expression by heat shock proteins Demonstration of heat shock protein 70-containing complexes of unactivated heat shock transcription factor 1. Protein Engineering 8(SUPPL ): 35, 1995

Protein phosphorylation in maize seedlings subjected to heat shock. Fiziologiya Rastenii (moscow): 1015-1018, 1988

An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene. Plant Physiology 121(3): 723-730, 1999

Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes to Cells 12(4): 487-499, 2007