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Major proteinase hydrolysing gliadin during wheat germination

, : Major proteinase hydrolysing gliadin during wheat germination. Phytochemistry (Oxford) 43(1): 39-44

A proteinase, representing the bulk of the enzyme activity for the hydrolysis of gliadin, was extracted from endosperms isolated from germinated seeds (four days) and was purified by ion-exchange chromatography the preparative isoelectric focusing. The optimal pH for gliadin hydrolysis was 4.25. The M-r, determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, was 30000; the isoelectric point was 4.5. The enzyme activity was totally inhibited by E-64 and cystatin, while inhibitors of other classes of proteinases were barely effective or ineffective. The activity was stimulated by sulphhydryl compounds. The proteinase hydrolysed to small peptides the gliadins from durum and soft wheat seeds. Other protein substrates were weakly degraded or not degraded. The proteinase appears to belong to the cysteine class and to play a key role in the initial mobilization of the main reserve protein in the starchy endosperm.

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Accession: 002889192

DOI: 10.1016/0031-9422(96)00193-8

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Gillette David D.; Gillette J.L.nett; Murphy Jack, 1985: The Tucumcari mammoths excavated by the Denver Museum of Natural History. Guidebook - New Mexico Geological Society 36: 14-31

Schwengers D., 1987: Glucooligosaccharide mixture and a process for its manufacture us patent 4649058 march 10 1987. Official Gazette Of The United States Patent & Trademark Office Patents2: 956

Romero M T.; Kepler K.L.; Bodnar R.J., 1988: Gender determinants of opioid mediation of swim analgesia in rats. Continuous cold-water swims (CCWS) and intermittent cold-water swims (ICWS) elicit respective nonopioid and opioid analgesic responses in adult male rats. The present experiment evaluated whether gender differences were observed in naloxone's...

Ingolfsson, A., 1982: Gulls, terns and skuas.. Fuglar Ritlandvernder, 8: 61-76

Wong, Gerard C. L., 2002: Self-assembled phases of biopolymers and counterions. Abstracts of Papers American Chemical Society 224(1-2): PMSE 36

Fujiwara H.; Tsuchida T.; Hamaoka T., 1980: Enhancement of lethal metastasis of plasma cytoma in mice rendered tolerant to tumor associated transplantation antigens. The role of tumor-specific immunity in the prevention of metastasis formation was investigated. C3H/He mice were nonspecifically immunosuppressed by total-body irradiation or T[thymus-derived]-cell depletion, or specifically immunodepressed by ren...

López Garciá, R.; Castelazo Morales, E.; Karchmer, S., 1983: Clinical validation of antepartum cardiotocographic tests. Ginecologia Y Obstetricia de Mexico 51(313): 121-130

Claypool, L.L.; Morris, L.L., 1952: Flying vegetables to market. Air transport of perishables demands good handling and packaging practices in all operations from the field to the consumer. Special care is required to prevent damage from air temps., altitude, and atmospheric pressures.