EurekaMag.com logo
+ Translate

Malpighian tubules of larval Aedes aegypti are hormonally stimulated by 5-hydroxytryptamine in response to increased salinity


, : Malpighian tubules of larval Aedes aegypti are hormonally stimulated by 5-hydroxytryptamine in response to increased salinity. Archives of Insect Biochemistry & Physiology 34(2): 123-141

Two environmental parameters, feeding status and salinity, are expected to affect water and ion balance of the aquatic larvae of Aedes aegypti. Evidence was obtained for regulation of Malpighian tubule fluid secretion rates in response to changes in each of these parameters. Exposure to increased salinity induces release into the hemolymph of material with diuretic effects on Malpighian tubules. Diuretic material is present in hemolymph of larvae raised in higher salinities, rapidly appears in the hemolymph of larvae following transfer from dilute water to higher salinity, and rapidly disappears from the hemolymph following transfer from higher salinity to dilute water. Feeding status affects diuretic properties of both hemolymph and Malpighian tubules. Feeding causes hemolymph to become diuretic relative to hemolymph from nonfeeding larvae. Malpighian tubules removed from feeding larvae have greater basal fluid secretion rates and also appear to have greater maximal fluid secretion capacity than do tubules removed from nonfeeding larvae. Larval hemolymph (5-HT) was found to increase fivefold in response to elevated salinity but was unaffected by feeding status. Methiothepin, a 5-HT receptor antagonist, inhibited stimulation of fluid secretion by 5-HT and blocked the diuretic effects of hemolymph from larvae exposed to higher salinity but was without effect on stimulation of fluid secretion by diuretic peptide. During the course of this investigation, a preliminary pharmacological characterization of the 5-HT receptor on Aedes Malpighian tubules, suggesting that this receptor may be pharmacologically distinct from other described insect 5-HT receptors, was obtained.

Accession: 002889555

DOI: 10.1002/(sici)1520-6327(1997)34:2<123::aid-arch1>3.0.co;2-y

Download PDF Full Text: Malpighian tubules of larval Aedes aegypti are hormonally stimulated by 5-hydroxytryptamine in response to increased salinity


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Veenstra, J.A., 1988: Effects of 5-hydroxytryptamine on the Malpighian tubules of Aedes aegypti. The effects of 5-hydroxytryptamine [serotonin], noradrenaline, adrenaline, octopamine and dopamine on the transepithelial voltage (TV) and fluid secretion of Malpighian tubules of A. aegypti were studied. Of these biogenic amines, only serotonin a...

Veenstra J.A.; Beyenbach K.W., 1986: Effects of 5 hydroxytryptamine on malpighian tubules of the mosquito aedes aegypti. Federation Proceedings 45(4): 759

Clark, T.M.; Bradley, T.J., 1996: Stimulation of Malpighian tubules from larval Aedes aegypti by secretagogues. Dibutyryl-cAMP (db-cAMP) and 5-hydroxytryptamine (5-HT) stimulate secretion rates of fluid, sodium, and potassium by Malpighian tubules from larval female Aedes aegypti, without affecting the sodium and potassium concentrations of the secreted flu...

Clark, T.M.; Bradley, T.J., 1992: Tissue extracts stimulate fluid secretion by larval Aedes aegypti Malpighian tubules. American Zoologist 32(5): 49A

Parrish A.; Petzel D.; Stanley Samuelson D., 1992: Prostaglandin e 2 stimulates increased intracellular camp concentrations in malpighian tubules of the yellow fever mosquito aedes aegypti. FASEB Journal 6(5): A1747

Vegni Talluri, M.; Cancrini, G., 1994: An ultrastructural study on the early cellular response to Dirofilaria immitis in the Malpighian tubules of Aedes aegypti. Dirofilaria immitis living in Aedes aegypti refractory strains were studied in relation to ultrastructural events in primary cells of Malpighian tubules and to defense mechanisms activated by hostcells. When the microfilaria reaches the Malpighian...

Vegni Talluri, M.; Cancrini, G., 1994: An ultrastructural study on the early cellular response to Dirofilaria immitis (Nematoda) in the Malpighian tubules of Aedes aegypti (refractory strains). Dirofilaria immitis in A. aegypti refractory strains were studied in relation to ultrastructural events in primary cells of Malpighian tubules and to defense mechanisms activated by host-cells. When the microfilaria reaches the Malpighian cells, i...

Beyenbach, K.W.; Oviedo, A.; Aneshansley, D.J., 1993: Malpighian tubules of Aedes aegypti: five tubules, one function. Urine formation in the yellow fever mosquito begins by secretion of Na, K, Cl and water in the distal, blind-ended portions of five Malpighian tubules. The tubules appear structurally similar. To answer the question whether the tubules are also fu...

Weng, X-He.; Piermarini, P.M.; Yamahiro, A.; Yu, M-Jiun.; Aneshansley, D.J.; Beyenbach, K.W., 2008: Gap junctions in Malpighian tubules of Aedes aegypti. We present electrical, physiological and molecular evidence for substantial electrical coupling of epithelial cells in Malpighian tubules via gap junctions. Current was injected into one principal cell of the isolated Malpighian tubule and membran...

Tulloch, G.S.; Goldman, M., 1942: The Malpighian tubules of Aedes aegypti L. (Diptera, Culicidae). Bulletin of the Brooklyn Entomological Society, 37: 52-56