EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice


Nucleic Acids Research 25(5): 1009-1014
Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice
The ovine beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a second gene construct comprising a mammalian cDNA or a CAT reporter sequence it falls to be expressed in the majority of transgenic lines generated. We suggest that mammalian cDNAs and prokaryotic reporter sequences can serve as active foci for gene silencing in the mammalian genome.


Accession: 002889579

PMID: 9023112

DOI: 10.1093/nar/25.5.1009



Related references

Reporter gene expression in cones in transgenic mice carrying bovine rhodopsin promoter/lacZ transgenes. Visual Neuroscience 11(6): 1227-1231, 1994

Expression of genomic and cDNA transgenes after co-integration in transgenic mice. Transgenic Research 4(1): 39-43, 1995

Characterization of pathology in transgenic mice over-expressing human genomic and cDNA tau transgenes. Neurobiology of Disease 7(2): 87-98, 2000

Reporter gene expression driven by human beta-PDE 5 sequences in transgenic mice. Investigative Ophthalmology & Visual Science 37(3): S337, 1996

Synapsin i 5 flanking sequences direct neuronal lacz reporter gene expression in transgenic mice. Society for Neuroscience Abstracts 17(1-2): 1512, 1991

Behavioral impairment in transgenic mice carrying both mutant APP and PS1 transgenes Comparison with singly transgenic APP and PS1 mice. Society for Neuroscience Abstracts 25(1-2): 1861, 1999

A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference (vol 33, pg 401, 2003). Nature Genetics 39(6): 803, 2007

Similarities and differences among inner retinal neurons revealed by the expression of reporter transgenes controlled by Brn-3a, Brn-3b, and Brn-3c promotor sequences. Visual Neuroscience 13(5): 955-962, 1996

Similarities and differences among inner retinal neurons revealed by the expression of reporter transgenes controlled by Brn-3a, Brn-3b, and Brn-3c promoter sequences. Visual Neuroscience 13(5): 955-962, 1996

Autofeedback suppression of growth hormone (GH) secretion in transgenic mice expressing a human GH reporter targeted by tyrosine hydroxylase 5'-flanking sequences to the hypothalamus. Endocrinology 136(9): 4044-4048, 1995