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Mammalian oocyte growth in vitro is stimulated by soluble factor(s) produced by preantral granulosa cells and by Sertoli cells


, : Mammalian oocyte growth in vitro is stimulated by soluble factor(s) produced by preantral granulosa cells and by Sertoli cells. Molecular Reproduction and Development 44(4): 540-546

Zona-free oocytes from 12-day-old mice were cultured on monolayers of NIH-3T3 fibroblasts, which enable the oocytes to establish gap junction communications with them, in the presence or absence of media conditioned by preantral granulosa cells or by Sertoli cells, plated at increasing concentrations from 0.3-1 x 106 cells per ml.

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Accession: 002889586

PMID: 8844697

DOI: 10.1002/(SICI)1098-2795(199608)44:4<540::AID-MRD14>3.0.CO;2-1

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