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Modified procedures for extraction and analysis of carrageenan applied to the red alga Hypnea musciformis



Modified procedures for extraction and analysis of carrageenan applied to the red alga Hypnea musciformis



Journal of Applied Phycology 7(6): 565-576



Dried powder of Hypnea musciformis was extracted with water at pH 7 after an initial short pre-treatment with cold, diluted HCl. Carrageenans were isolated by alcohol precipitation after an amylase treatment and a filtration of the extracts. The yields at 25 and 90 degree C were 25 and 75% (w/w) of the dry alga, with molecular weights (M-W) corresponding to 194 000 and 245 000, respectively. The chemical structure was dominated by G4S-DA-(kappa-carrageenan or carrageenose 4'-sulphate). A simple fractionation procedure for kappa-carrageenase hydrolysates, based on stirring in different ethanol/water mixtures, is introduced. NMR analysis showed that oligosaccharides with a repeating DA-G4S structure were the main constituents in the enzymic hydrolysates of the carrageenans from Hypnea musciformis. These oligosaccharides were solubilized in an ethanol concentration from 96 to 48% (v/v). In some enzyme resistant fractions D6S-G4S and DA2S-G4S sequences and D2S,6S units were detected by 13C-NMR.

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Accession: 002897692

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DOI: 10.1007/bf00003944



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