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Molecular cloning and expression of the coat protein genes of Cf, a filamentous bacteriophage of Xanthomonas campestris pv. citri

Yang MeiKwei; Huang HueiMei; Yang YenChun; Su WeiChih

Botanical Bulletin of Academia Sinica 36(4): 207-214

1995


Accession: 002898365

Particles of the filamentous bacteriophage Cf contain a major coat protein, the B protein, with a MW of approx. 6000. In addition, a minor coat protein, the A protein, with a MW of c. 50 000, was also identified on sodium dodecyl sulfate-containing polyacrylamide gels. A 3.3 kbp HincII fragment derived from Cf genome was cloned into the expression plasmid pG308N, an E. coli plasmid which carries the pL promoter.

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