Morphological and molecular characterization of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop in Brazil
Kuramae Izioka, E.E.; Lopes, C.R.; Souza, N.L.; Machado, M.A.
European Journal of Plant Pathology 103(4): 323-329
ISSN/ISBN: 0929-1873 DOI: 10.1023/a:1008627302706
Brazilian isolates of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop were examined for colony colour, mycelial growth, benomyl-resistance, pathogenicity and genetic variability by random amplified polymorphic DNA (RAPD) analysis. All isolates were obtained from flowers and persistent calyxes from different citrus hosts from Sao Paulo, Brazil. DNA polymorphisms detected after amplification with random 10-mer primers were used to classify the isolates into 2 groups.