EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Multi-target and medium-independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere



Multi-target and medium-independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere



FEMS Microbiology Ecology 22(3): 183-192



Glucanolytic bacteria from barley rhizosphere soil were isolated by a procedure selecting for isolates with beta-glucosidase activity. Almost all isolates were fast-growing, Gram-positive rods. Sixteen out of 100 isolates showed in vitro fungal antagonism against widely different plant-pathogenic microfungi (Aphanomyces cochleoides, Pythium ultimum and Rhizoctonia solani). The 16 isolates shared a characteristic profile of cell-wall-degrading enzymes, comprising glucanolytic (cellulase, mannanase and xylanase) and proteolytic enzymes. This enzyme profile was not observed in any of the non-antagonistic isolates and may thus be useful in selection protocols for fungal antagonists producing fungal cell-wall-degrading enzymes. The antagonistic isolates were identified as Paenibacillus (Bacillus) polymyxa (2 strains) and Bacillus pumilus (13 strains), Bacillus sp. (1 strain). Both P. polymyxa isolates and a subgroup of 4 B. pumilus isolates showed a medium-independent antagonism as tested against A. cochleoides in four different media. This was apparently related to constitutive production of cell-wall-degrading enzymes (P. polymyxa) or induction of a repertoire of enzymes substituting for each other in different media (B. pumilus). Despite different strategies of enzyme production in the media, both the P. polymyxa and B. pumilus strains demonstrate a multi-target and medium-independent type of fungal antagonism, which is promising for application in biological control.

(PDF emailed within 0-6 h: $19.90)

Accession: 002900831

Download citation: RISBibTeXText

DOI: 10.1016/s0168-6496(96)00089-x



Related references

Paenibacillus polymyxa antibiotics, hydrolytic enzymes and hazard assessment. Journal Of Plant Pathology: 3, 419-430, 2008

Detection and quantification of Paenibacillus polymyxa in the rhizosphere of wild barley (Hordeum spontaneum) with real-time PCR. Journal of Applied Microbiology 107(3): 736-745, 2010

Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil. Research in Microbiology 151(5): 369-381, June, 2000

Isolation of Bacillus circulans and Paenibacillus polymyxa strains inhibitory to Campylobacter jejuni and characterization of associated bacteriocins. Journal of Food Protection 68(1): 11-17, 2005

Cytometric monitoring of growth, sporogenesis and spore cell sorting in Paenibacillus polymyxa (formerly Bacillus polymyxa). Journal of Applied Microbiology 92(3): 475-481, 2002

Cell wall teichoic acids of Bacillus licheniformis VKM B-511T, Bacillus pumilus VKM B-58T, and other strains previously assigned to Bacillus pumilus. 2012

Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. Letters in Applied Microbiology 43(6): 625-630, 2006

Some oxidative processes in strains of Bacillus subtilis and Bacillus mesentericus- producers of hydrolytic enzymes. Mikrobiol Zh Akad Nauk Ukr Rsr 28(3): 3-8, 1966

Draft Genome Sequences of Paenibacillus polymyxa NRRL B-30509 and Paenibacillus terrae NRRL B-30644, Strains from a Poultry Environment That Produce Tridecaptin A and Paenicidins. Genome Announcements 3(2): -, 2015

Diversity of Paenibacillus polymyxa populations in the rhizosphere of wheat (Triticum durum) in Algerian soils. European Journal of Soil Biology 36(3/4): 149-159, 2000

Disruption of the Paenibacillus polymyxa levansucrase gene impairs its ability to aggregate soil in the wheat rhizosphere. Environmental Microbiology 2(3): 333-342, 2000

Disruption of the Paenibacillus polymyxa levansucrase gene impairs its ability to aggregate soil in the wheat rhizosphere. Environmental Microbiology 2(3): 333-342, 2001