+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi



Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi



Journal of Clinical Microbiology 35(1): 139-143



A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizus. The effect of inoculum density (approximately 10(3) and 10(4) CFU/ml), incubation time (24, 48, and 72 h), and procedure of MIC determination (conventional and colorimetric [Alamar Blue] evaluation of growth inhibition) on intra- and interlaboratory agreement was analyzed. Based on intra- (97 to 100%) and interlaboratory (94 to 95%) agreement for both drugs, the overall optimal testing conditions identified were determination of colorimetric MICs after 48 to 72 h of incubation with an inoculum density of approximately 10(4) CFU/ml. These testing conditions are proposed as guidelines for a reference broth microdilution method.

(PDF emailed within 1 workday: $29.90)

Accession: 002900845

Download citation: RISBibTeXText

PMID: 8968895


Related references

Multicenter evaluation of standardized inoculum for antifungal susceptibility testing of filamentous fungi. Abstracts of the General Meeting of the American Society for Microbiology 93(0): 529, 1993

Multicenter evaluation of the reproducibility of the proposed antifungal susceptibility testing method for fermentative yeasts of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST). Clinical Microbiology and Infection 9(6): 467-474, 2003

Second NCCLS evaluation of antifungal susceptibility testing of filamentous fungi. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 35(0): 73, 1995

Evaluation of Etest for antifungal susceptibility testing of filamentous fungi. Abstracts of the General Meeting of the American Society for Microbiology 96(0): 85, 1996

Multicentre determination of quality control strains and quality control ranges for antifungal susceptibility testing of yeasts and filamentous fungi using the methods of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST). Clinical Microbiology and Infection 13(10): 1018-1022, 2007

Comparative evaluation of alternative methods for antifungal susceptibility testing of filamentous fungi. Infeksiyon Dergisi = Turkish Journal of Infection 12(3): 407-411, 1998

Standardizing antifungal susceptibility testing for filamentous fungi A comparative and collaborative evaluation. Abstracts of the General Meeting of the American Society for Microbiology 93(0): 528, 1993

Comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. Antimicrobial Agents and ChemoTherapy 39(2): 314-319, 1995

Multicenter evaluation of the proposed EUCAST antifungal susceptibility testing method. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 41: 372, 2001

Evaluation of antifungal susceptibility testing for filamentous fungi assayed by broth microdilution and Etest. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 38: 483, 1998

Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi. Journal of Antimicrobial ChemoTherapy 50(5): 719-722, 2002

Susceptibility of yeast and some filamentous fungi to antifungal agents by a standardized micromethod. Bulletin de la Societe Francaise de Mycologie Medicale 16(2): 395-398, 1987

An evaluation of different inoculum densities, incubation periods and MIC endpoint determination methods for antifungal susceptibility testing of filamentous fungi. Abstracts of the General Meeting of the American Society for Microbiology 95(0): 104, 1995

Evaluation of disk diffusion and Etest compared to broth microdilution for antifungal susceptibility testing of posaconazole against clinical isolates of filamentous fungi. Journal of Clinical Microbiology 45(4): 1322-1324, 2007

Evaluation of Etest performed in Mueller-Hinton agar supplemented with glucose for antifungal susceptibility testing of clinical isolates of filamentous fungi. Mycopathologia 177(3-4): 157-166, 2014