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Oxidants increase intracellular free Zn2+ concentration in rabbit ventricular myocytes

, : Oxidants increase intracellular free Zn2+ concentration in rabbit ventricular myocytes. American Journal of Physiology 272(5 Pt 2): H2095-H2106

Oxidative stress may alter cardiac function by affecting intracellular free Zn2+ concentrations ([Zn2+]i). Rabbit ventricular myocytes loaded with fura 2 were used to fluorometrically measure resting [Zn2+]i (0.23 [plus or minus] 0.03 nM) and intracellular Ca2+ concentration ([Ca2+]i) (36 [plus or minus] 7 nM). Fluorescence quenching by the heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine was used to quantitate [Zn2+]i. The thiol-reactive oxidants hypochlorous acid (0.1 mM) and selenite (1 mM) increased [Zn2+]i to 7.7 [plus or minus] 1.7 and 6.1 [plus or minus] 1.7 nM, respectively, within 5 min. Dithiothreitol (0.5 mM), a disulfide-reducing agent, rapidly restored normal [Zn2+]i. The oxidants did not affect [Ca2+]i. However, depolarization-induced Ca2+ transients and Ca2+ currents were zinc dependent. [Zn2+]i-associated fluorescence was substantial and, if ignored, it led to overestimation of [Ca2+]i by approximately twofold before oxidant treatment and by approximately eightfold after oxidants. The results demonstrate that [Zn2+]i 1) can be greatly increased by thiol-reactive oxidants; 2) may contribute to oxidant-induced alterations of excitation-contraction coupling; and 3) has strong fura 2 fluorescence which, if overlooked, can lead to significant overestimation of [Ca2+]i. Reprinted by permission of the publisher.

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Accession: 002914062

PMID: 9176274

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Eckert, John., 2001: First record of the black-faced monarch in South Australia. South Australian Ornithologist. May; 337: 139

Wolffgang, H., 1962: Data and ideas on the structure of plant viruses.. A survey of the literature from Inst. Phytopath., Aschersleben, Germany, is followed by a discussion, with particular consideration of tobacco mosaic virus and other viruses, including tobacco rattle, beet yellows, and rye-grass mosaic viruses, cu...

Atanasov, K.; Yovkova, T.; Tzvetkova, T., 1978: The effect of heparin and acetysal on blood platelet functions in coronary sclerosis. Folia Medica 20(1): 31-34

Tsubota, K.; Yamada, M.; Kajiwara, K.; Ugajin, S.; Hasegawa, T.; Kobayashi, T.K., 1992: Cytologic evaluation of conjunctival epithelium after cataract surgery. Although numerous studies have examined corneal epithelial wound healing, few reports describe conjunctival wound healing. Thus, we evaluated the conjunctival epithelium by brush cytology, in which a miniature brush was used to collect many cells...

Telander, R.L.; Lennox, C.; Sieber, W., 1976: Sequestration of the lung in children. Nine cases of sequestration of the lung were seen in children. Bronchopulmonary sequestration of the lung is a distinct congenital anomaly and clinical entity that can be distinguished from other congenital abnormalities of the lung. Such a congen...

Maynord Stephen T., 1994: Steep stream riprap design. Miscellaneous Paper HL (Vicksburg) Pages 8

Fries, G.G.nzlin, G., 1969: Estimation of beer hazes. Brauwelt 26, 109 (24 25) 454-456

Aguero R.M.; Pico G.; Guibert E.; Corchs J.L., 1987: Interaction of the organic anion 1 anilin0 8 naphthalenesulfonate ans with isolated rat hepatocytes. 1. The interaction of ANS with rat hepatocytes in time was studied by fluorescence spectroscopy. 2. The intercept of the first linear portion of the time curve of interaction showed a positive value over all the ANS concentration range employed. 3...

Vuorinen, P.; Sysimetsä, E., 1966: Indications for splenoportal venography with reference to the commonest clinical causes of portal hypertension. Annales Medicinae Internae Fenniae 55(2): 49-56

Petanceska, S.S.; Nagy, V.; Frail, D.; Gandy, S., 2000: Ovariectomy and 17beta-estradiol modulate the levels of Alzheimer's amyloid beta peptides in brain. Objective: To test whether female gonadal hormone status and estrogen modulate the metabolism of Abeta peptides in vivo. Background: AD is a neurodegenerative disorder characterized by accumulation of aggregated forms of the 40- and 42-amino acid...