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Oxygen-dependent increase of antioxidants in soybean embryonic axes


, : Oxygen-dependent increase of antioxidants in soybean embryonic axes. International Journal of Biochemistry & Cell Biology 27(11): 1221-1229

Oxygen, although essential for the survival of aerobic organisms leads to generation of toxic species. The effect of oxygen on enzymatic and non-enzymatic antioxidants was determined to evaluate response to oxidative stress in soybean axes. Soybean seeds were incubated over nutrient solution-saturated filter paper. Different oxygen concentrations in the incubation atmosphere were maintained by gassing either N-2 (0% O-2), air (20% O-2), a commercial mix 40% O-2 +- 60% N-2 (40% O-2) or O-2 (100% O-2) in closed plastic chambers. Oxidative stress was assessed by the oxidation of 2',7'-dichlorofluorescein diacetate. The activities of antioxidant enzymes were determined spectrophotometrically. alpha-Tocopherol and ubiquinol-10 contents were measured by HPLC. The weight of axes was 13 +- 1 and 27 +- 3 mg/axis in the absence and presence of 20% oxygen, respectively. 2',7-Dichlorofluorescein diacetate oxidation was increased from 14 +- 2 to 66 +- 5 AU/min/mg FW by supplementation of 20% oxygen. Total glutathione content was 22 +- 6 and 33 +- 6 nmol/axis in axes grown in absence of oxygen and air, respectively. Ubiquinol-10 content was not affected by oxygen. alpha-Tocopherol content decreased from 384 +- 94 to 14 +- 3 pmol/axis in the absence or presence of 100% oxygen, respectively. The activities of antioxidant enzymes increased in axes exposed to oxygen. Our data suggest that exposure of soybean axes to oxygen leads to oxidative stress but damage by oxygen intermediates was limited by increases in the activity of both, antioxidant substances (i.e. glutathione) and antioxidant enzymes.

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Accession: 002914209

DOI: 10.1016/1357-2725(95)00086-5

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