+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxygen-dependent increase of antioxidants in soybean embryonic axes

, : Oxygen-dependent increase of antioxidants in soybean embryonic axes. International Journal of Biochemistry & Cell Biology 27(11): 1221-1229

Oxygen, although essential for the survival of aerobic organisms leads to generation of toxic species. The effect of oxygen on enzymatic and non-enzymatic antioxidants was determined to evaluate response to oxidative stress in soybean axes. Soybean seeds were incubated over nutrient solution-saturated filter paper. Different oxygen concentrations in the incubation atmosphere were maintained by gassing either N-2 (0% O-2), air (20% O-2), a commercial mix 40% O-2 +- 60% N-2 (40% O-2) or O-2 (100% O-2) in closed plastic chambers. Oxidative stress was assessed by the oxidation of 2',7'-dichlorofluorescein diacetate. The activities of antioxidant enzymes were determined spectrophotometrically. alpha-Tocopherol and ubiquinol-10 contents were measured by HPLC. The weight of axes was 13 +- 1 and 27 +- 3 mg/axis in the absence and presence of 20% oxygen, respectively. 2',7-Dichlorofluorescein diacetate oxidation was increased from 14 +- 2 to 66 +- 5 AU/min/mg FW by supplementation of 20% oxygen. Total glutathione content was 22 +- 6 and 33 +- 6 nmol/axis in axes grown in absence of oxygen and air, respectively. Ubiquinol-10 content was not affected by oxygen. alpha-Tocopherol content decreased from 384 +- 94 to 14 +- 3 pmol/axis in the absence or presence of 100% oxygen, respectively. The activities of antioxidant enzymes increased in axes exposed to oxygen. Our data suggest that exposure of soybean axes to oxygen leads to oxidative stress but damage by oxygen intermediates was limited by increases in the activity of both, antioxidant substances (i.e. glutathione) and antioxidant enzymes.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002914209

DOI: 10.1016/1357-2725(95)00086-5

Download PDF Full Text: Oxygen-dependent increase of antioxidants in soybean embryonic axes

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Mitta A.E.A.; Pozzi O.; Arciprete C.P.; Gros E.G., 1988: An improved procedure for the synthesis of 3 iodo 2 4 6 trimethylphenylcarbamoylmethyliminodiacetic acid an efficient complex agent for technetium 99m. The synthesis of 3-iodo-2, 4, 6-trimethylphenyl-carbamoylmethyl-iminodiacetic acid by a modified procedure is described. Its spectroscopic properties as well as its 99mTc complexing capacity are also presented.

KEENEY, E.L., 2011: Sodium caprylate; a new and effective treatment for moniliasis of the skin and mucous membranes. Bulletin of the Johns Hopkins Hospital 78: 333-339

Engeszer, R.E.; Patterson, L.B.; Rao, A.A.; Parichy, D.M., 2007: Zebrafish in the wild: a review of natural history and new notes from the field. The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats, and life cycle of t...

Mathias, J.M., 2010: Automation ends preop paper chase. Or Manager 25(6): 11-2, 14

Bodin, I.; Julin, P.; Thomsson, M., 1981: Hyperodontia. III. Supernumerary anterior teeth. Dento Maxillo Facial Radiology 10(1): 35-42

Gale, C.R.; Martyn, C.N., 1995: Dummies and the health of Hertfordshire infants, 1911-1930. Social History of Medicine 8(2): 231-255

Halkin, A.; Rosenschein, U.; Barron, H.V.; Viskin, S., 2002: Prevention of cardiac arrest: risk assessment after myocardial infarction. Cardiac Electrophysiology Review 6(1-2): 9-14

Arnull, A. M., 1984: Judicial review of economic discretion in the European Community. This is an analysis of the attitude of the Court of Justice to the exercise by the Council and Commission of discretionary powers in the economic sphere, particularly in the field of agriculture.

Kobori, M.; Miyama, Y.; Tsushida, T.; Shinmoto, H.; Shinohara, K., 1995: Effect of non-dialyzable extracts of vegetables on the differentiation of U-937 human myeloid leukemia cell line. The effects of non-dialyzable extracts of several vegetables such as eggplant, green pepper, cabbage, Garland chrysanthemum, kidney beans, cucumber and Welsh onion on the differentiation of U-937 human myeloid leukemia cells were examined. Among t...

Huang, L.; Ponomarenko, N.; Wiederrecht, G.P.; Tiede, D.M., 2012: Cofactor-specific photochemical function resolved by ultrafast spectroscopy in photosynthetic reaction center crystals. High-resolution mapping of cofactor-specific photochemistry in photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides was achieved by polarization selective ultrafast spectroscopy in single crystals at cryogenic temperature. By exploit...